Maternal transfer and sublethal immune system effects of brevetoxin exposure in nesting loggerhead sea turtles (Caretta caretta) from western Florida
| Autor: | Taylor M. Greenan, Katherine D. Bauman, Catherine J. Walsh, Justin R. Perrault, Michael S. Henry, Patricia Blum |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
food.ingredient Embryo Nonmammalian Health Toxicology and Mutagenesis Red tide Harmful Algal Bloom Zoology Enzyme-Linked Immunosorbent Assay 010501 environmental sciences Aquatic Science 01 natural sciences law.invention Brevetoxin food law Tandem Mass Spectrometry Yolk Animals Water Pollutants Turtle (robot) Hatchling Chromatography High Pressure Liquid 0105 earth and related environmental sciences Ovum Yolk Sac biology Reproductive success Ecology 010604 marine biology & hydrobiology Reproduction Oxocins Dinoflagellate Environmental Exposure biology.organism_classification Reactive Nitrogen Species Immunity Innate Turtles Oxidative Stress Dinoflagellida Florida Female Marine Toxins Karenia brevis Reactive Oxygen Species |
| Zdroj: | Aquatic toxicology (Amsterdam, Netherlands). 180 |
| ISSN: | 1879-1514 |
| Popis: | Blooms of Karenia brevis (also called red tides) occur almost annually in the Gulf of Mexico. The health effects of the neurotoxins (i.e., brevetoxins) produced by this toxic dinoflagellate on marine turtles are poorly understood. Florida's Gulf Coast represents an important foraging and nesting area for a number of marine turtle species. Most studies investigating brevetoxin exposure in marine turtles thus far focus on dead and/or stranded individuals and rarely examine the effects in apparently "healthy" free-ranging individuals. From May-July 2014, one year after the last red tide bloom, we collected blood from nesting loggerhead sea turtles (Caretta caretta) on Casey Key, Florida USA. These organisms show both strong nesting and foraging site fidelity. The plasma was analyzed for brevetoxin concentrations in addition to a number of health and immune-related parameters in an effort to establish sublethal effects of this toxin. Lastly, from July-September 2014, we collected unhatched eggs and liver and yolk sacs from dead-in-nest hatchlings from nests laid by the sampled females and tested these samples for brevetoxin concentrations to determine maternal transfer and effects on reproductive success. Using a competitive enzyme-linked immunosorbent assay (ELISA), all plasma samples from nesting females tested positive for brevetoxin (reported as ng brevetoxin-3[PbTx-3] equivalents [eq]/mL) exposure (2.1-26.7ng PbTx-3eq/mL). Additionally, 100% of livers (1.4-13.3ng PbTx-3eq/mL) and yolk sacs (1.7-6.6ng PbTx-3eq/mL) from dead-in-nest hatchlings and 70% of eggs ( |
| Databáze: | OpenAIRE |
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