A Study of DNA, Partially Denatured DNA and Protein-DNA Complexes in the Polyethyleneglycol-Dextran Phase System
| Autor: | D. Pettijohn |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
DNA
Bacterial Hot Temperature Chemical Phenomena Buffers Nucleic Acid Denaturation Tritium medicine.disease_cause Coliphages Biochemistry Glycols chemistry.chemical_compound Nucleic acid thermodynamics RNA polymerase Centrifugation Density Gradient Escherichia coli medicine Chymotrypsin Denaturation (biochemistry) Carbon Isotopes biology Chemistry Physical Phosphorus Isotopes RNA Dextrans RNA Nucleotidyltransferases Crystallography chemistry DNA Viral biology.protein Polyomavirus DNA |
| Zdroj: | European Journal of Biochemistry. 3:25-32 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1967.tb19495.x |
| Popis: | Partition properties of DNA and protein-DNA complexes in the polyethyleneglycol-dextran phase system are described which suggest several preparative and possibly analytical applications. The partition coefficients (K) of linear and circular double-stranded DNAs are significantly reduced if relatively small region(s) of local denaturation are introduced into the DNA. The K value of polyoma DNA I, the twisted circular form, is approximately 10 fold less than the values of II and III, the open circular and linear DNA forms. DNA complexed with several different basic proteins or polyamines did not partition differently from free DNA; however, the complex with chymotrypsin or RNA polymerase had greatly reduced K values. The saturated RNA polymerase-DNA complex was indistinguishable from heat denatured DNA in its partition properties. |
| Databáze: | OpenAIRE |
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