DspA/E, a type III effector essential for Erwinia amylovora pathogenicity and growth in planta, induces cell death in host apple and nonhost tobacco plants
| Autor: | Hayat El-Maarouf-Bouteau, Igor Pucheu, Marie-Noëlle Brisset, Marie-Anne Barny, Tristan Boureau, Amélie Garnier, Claude Perino |
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| Přispěvatelé: | Pathologie Végétale (PaVé), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Unité de recherche Pathologie végétale et phytobactériologie, Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2006 |
| Předmět: |
Hypersensitive response
Time Factors Physiology Nicotiana tabacum PROTEINE DSPA/E Gene Expression PATHOGENICITE Erwinia Microbiology Type three secretion system 03 medical and health sciences chemistry.chemical_compound Necrosis Bacterial Proteins Tobacco Erwinia amylovora [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Secretion Amino Acid Sequence RNA Messenger Phylogeny 030304 developmental biology Plant Diseases Plant Proteins 0303 health sciences biology Cell Death 030306 microbiology Effector fungi Callose General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Plant Leaves chemistry Malus Fire blight Mutation Agronomy and Crop Science Sequence Alignment Rhizobium |
| Zdroj: | Molecular Plant-Microbe Interactions Molecular Plant-Microbe Interactions, American Phytopathological Society, 2006, 19 (1), pp.16-24. ⟨10.1094/MPMI-19-0016⟩ |
| ISSN: | 0894-0282 |
| Popis: | Erwinia amylovora is responsible for fire blight, a necrotic disease of apples and pears. E. amylovora relies on a type III secretion system (TTSS) to induce disease on hosts and hypersensitive response (HR) on nonhost plants. The DspA/E protein is essential for E. amylovora pathogenicity and is secreted via the TTSS in vitro. DspA/E belongs to a type III effector family that is conserved in several phytopathogenic bacteria. In E. amylovora, DspA/E has been implicated in the generation of an oxidative stress during disease and the suppression of callose deposition. We investigated the fate of DspA/E in planta. DspA/E delivered artificially to apple or tobacco cells by agroinfection induced necrotic symptoms, indicating that DspA/E was probably injected via the TTSS. We confirmed that DspA/E acts as a major cell-death inducer during disease and HR, because the dspA/E mutant is severely impaired in its ability to induce electrolyte leakage in apple and tobacco leaves. Expression of the defense marker gene PR1 was delayed when dspA/E was transiently expressed in tobacco, suggesting that DspA/E-mediated necrosis may be associated with an alteration of defense responses. |
| Databáze: | OpenAIRE |
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