Folinic acid increases protein arginine methylation in human endothelial cells
| Autor: | Pieter Koolwijk, Ruben Esse, Tom Teerlink, Rita Castro, Isabel Tavares de Almeida, Henk J. Blom |
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| Přispěvatelé: | VU University medical center, Physiology, ACS - Microcirculation |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Hyperhomocysteinemia Methyltransferase Homocysteine Arginine Leucovorin lcsh:TX341-641 folate Methylation Article 03 medical and health sciences chemistry.chemical_compound Methionine Internal medicine medicine Human Umbilical Vein Endothelial Cells Humans Cells Cultured Nutrition and Dietetics Dose-Response Relationship Drug cellular methylation capacity homocysteine medicine.disease S-Adenosylhomocysteine 3. Good health 030104 developmental biology Endocrinology chemistry protein arginine methylation lcsh:Nutrition. Foods and food supply Protein Processing Post-Translational Homeostasis Intracellular Food Science |
| Zdroj: | Nutrients, 10(4):404. Multidisciplinary Digital Publishing Institute (MDPI) Esse, R, Teerlink, T, Koolwijk, P, Tavares de Almeida, I, Blom, H J & Castro, R 2018, ' Folinic acid increases protein arginine methylation in human endothelial cells ', Nutrients, vol. 10, no. 4, 404 . https://doi.org/10.3390/nu10040404 Nutrients Nutrients; Volume 10; Issue 4; Pages: 404 Nutrients, Vol 10, Iss 4, p 404 (2018) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu10040404 |
| Popis: | Elevated plasma total homocysteine (tHcy) is associated with increased risk of cardiovascular disease, but the mechanisms underlying this association are not completely understood. Cellular hypomethylation has been suggested to be a key pathophysiologic mechanism, since S-adenosylhomocysteine (AdoHcy), the Hcy metabolic precursor and a potent inhibitor of methyltransferase activity, accumulates in the setting of hyperhomocysteinemia. In this study, the impact of folate and methionine on intracellular AdoHcy levels and protein arginine methylation status was studied. Human endothelial cells were incubated with increasing concentrations of folinic acid (FnA), a stable precursor of folate, with or without methionine restriction. The levels of intracellular AdoHcy and AdoMet, tHcy in the cell culture medium, and protein-incorporated methylarginines were evaluated by suitable liquid chromatography techniques. FnA supplementation, with or without methionine restriction, reduced the level of tHcy and did not affect intracellular AdoMet levels. Interestingly, FnA supplementation reduced intracellular AdoHcy levels only in cells grown under methionine restriction. Furthermore, these cells also displayed increased protein arginine methylation status. These observations suggest that folic acid supplementation may enhance cellular methylation capacity under a low methionine status. Our results lead us to hypothesize that the putative benefits of folic acid supplementation in restoring endothelial homeostasis, thus preventing atherothrombotic events, should be reevaluated in subjects under a methionine restriction diet. |
| Databáze: | OpenAIRE |
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