Plasma modified PLA electrospun membranes for actinorhodin production intensification in Streptomyces coelicolor immobilized-cell cultivations
| Autor: | Alberto Sutera, Giuseppe Gallo, Roberto Scaffaro, Luigi Botta, Francesco Lopresti, RM Fontana |
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| Přispěvatelé: | Scaffaro, R., Lopresti, F., Sutera, A., Botta, L., Fontana, R., Gallo, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Polyesters Segmented filamentous bacteria S. coelicolor immobilization Anthraquinones Streptomyces coelicolor 02 engineering and technology Secondary metabolite Settore BIO/19 - Microbiologia Generale Actinorhodin Microbiology 03 medical and health sciences chemistry.chemical_compound Colloid and Surface Chemistry stomatognathic system Polylactic acid medicine Plasma treatment Physical and Theoretical Chemistry Incubation Mycelium biology Electrospinning Photoelectron Spectroscopy Prodigiosin Actinorhodin production technology industry and agriculture Settore ING-IND/34 - Bioingegneria Industriale PLA membrane Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Anti-Bacterial Agents 030104 developmental biology Membrane Settore ING-IND/22 - Scienza E Tecnologia Dei Materiali chemistry Biochemistry Microscopy Electron Scanning 0210 nano-technology Biotechnology medicine.drug |
| Popis: | Most of industrially relevant bioproducts are produced by submerged cultivations of actinomycetes. The immobilization of these Gram-positive filamentous bacteria on suitable porous supports may prevent mycelial cell-cell aggregation and pellet formation which usually negatively affect actinomycete submerged cultivations, thus, resulting in an improved biosynthetic capability. In this work, electrospun polylactic acid (PLA) membranes, subjected or not to O2-plasma treatment (PLA-plasma), were used as support for immobilized-cell submerged cultivations of Streptomyces coelicolor M145. This strain produces different bioactive compounds, including the blue-pigmented actinorhodin (ACT) and red-pigmented undecylprodigiosin (RED), and constitutes a model for the study of antibiotic-producing actinomycetes. Wet contact angles and X-ray photoelectron spectroscopy analysis confirmed the increased wettability of PLA-plasma due to the formation of polar functional groups such as carboxyl and hydroxyl moieties. Scanning electron microscope observations, carried out at different incubation times, revealed that S. coelicolor immobilized-cells created a dense âbiofilm-likeâ mycelial network on both kinds of PLA membranes. Cultures of S. coelicolor immobilized-cells on PLA or PLA-plasma membranes produced higher biomass (between 1.5 and 2 fold) as well as higher levels of RED and ACT than planktonic cultures. In particular, cultures of immobilized-cells on PLA and PLA-plasma produced comparable levels of RED that were approximatively 4 and 5 fold higher than those produced by planktonic cultures, respectively. In contrast, levels of ACT produced by immobilized-cell cultures on PLA and PLA-plasma were different, being 5 and 10 fold higher than those of planktonic cultures, respectively. Therefore, this is study demonstrated the positive influence of PLA membrane on growth and secondary metabolite production in S. coelicolor and also revealed that O2-plasma treated PLA membranes specifically promoted higher ACT production than not treated membranes. |
| Databáze: | OpenAIRE |
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