Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma viride
| Autor: | Michiki Aso, Ryouichi Tanaka, Renon Matsumae, Ayumi Tanaka, Atsushi Takabayashi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Lineage (evolution) Photosynthetic Reaction Center Complex Proteins Light-Harvesting Protein Complexes Single group Plant Science 01 natural sciences Mesostigma Mass Spectrometry 03 medical and health sciences Algae Centrifugation Density Gradient Phylogeny Photosystem Gel electrophoresis biology Phylogenetic tree Photosystem I Protein Complex Chemistry Photosystem II Protein Complex Cell Biology General Medicine Sequence Analysis DNA biology.organism_classification 030104 developmental biology Biochemistry Green algae Electrophoresis Polyacrylamide Gel Streptophyta 010606 plant biology & botany |
| Zdroj: | Plantcell physiology. 62(3) |
| ISSN: | 1471-9053 |
| Popis: | Land plants evolved from a single group of streptophyte algae. One of the key factors needed for adaptation to a land environment is the modification in the peripheral antenna systems of photosystems (PSs). Here, the PSs of Mesostigma viride, one of the earliest-branching streptophyte algae, were analyzed to gain insight into their evolution. Isoform sequencing and phylogenetic analyses of light-harvesting complexes (LHCs) revealed that M. viride possesses three algae-specific LHCs, including algae-type LHCA2, LHCA9 and LHCP, while the streptophyte-specific LHCB6 was not identified. These data suggest that the acquisition of LHCB6 and the loss of algae-type LHCs occurred after the M. viride lineage branched off from other streptophytes. Clear-native (CN)-polyacrylamide gel electrophoresis (PAGE) resolved the photosynthetic complexes, including the PSI–PSII megacomplex, PSII–LHCII, two PSI–LHCI–LHCIIs, PSI–LHCI and the LHCII trimer. Results indicated that the higher-molecular weight PSI–LHCI–LHCII likely had more LHCII than the lower-molecular weight one, a unique feature of M. viride PSs. CN-PAGE coupled with mass spectrometry strongly suggested that the LHCP was bound to PSII–LHCII, while the algae-type LHCA2 and LHCA9 were bound to PSI–LHCI, both of which are different from those in land plants. Results of the present study strongly suggest that M. viride PSs possess unique features that were inherited from a common ancestor of streptophyte and chlorophyte algae. |
| Databáze: | OpenAIRE |
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