Staphylococcal infection prevention using antibiotic‐loaded mannitol–chitosan paste in a rabbit model of implant‐associated osteomyelitis
| Autor: | J. Amber Jennings, Karen E. Beenken, Joel D. Bumgardner, Mark S. Smeltzer, Zoe L. Harrison, Leslie Pace, Madison N. Brown, Warren O. Haggard |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0206 medical engineering
Biocompatible Materials 02 engineering and technology medicine.disease_cause Bone tissue Article Polyethylene Glycols Microbiology Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Vancomycin medicine Animals Mannitol Orthopedics and Sports Medicine Titanium 030203 arthritis & rheumatology technology industry and agriculture Biomaterial Osteomyelitis Staphylococcal Infections Bone cement 020601 biomedical engineering Anti-Bacterial Agents medicine.anatomical_structure chemistry Amikacin Staphylococcus aureus Rabbits medicine.drug |
| Zdroj: | J Orthop Res |
| ISSN: | 1554-527X 0736-0266 |
| DOI: | 10.1002/jor.24986 |
| Popis: | Antibiotic-loaded chitosan pastes have shown advantages in treatment and coverage of complex musculoskeletal defects. We added mannitol, previously shown to increase antibiotic susceptibility of biofilm, to an injectable chitosan/polyethylene glycol paste for delivery of antibiotics. Ground sponges (0.85% acetic acid solution, 1% chitosan, 0% or 2% mannitol, 1% polyethylene glycol) were hydrated using phosphate buffered saline with 10 mg/ml amikacin and 10 mg/ml vancomycin added to form pastes. We inoculated rabbit radial defects with 105 colony forming units of Staphylococcus aureus (UAMS-1) and inserted titanium pins into cortical bone. Groups compared included mannitol blend pastes, non-mannitol blends, antibiotic-loaded bone cement, vancomycin powder, and no treatment controls. We harvested tissue samples and retrieved the pins retrieved at 3 weeks. All antibiotic-loaded groups lowered bacterial growth and CFU counts in soft and bone tissue and on titanium pins in in vivo studies. Results indicate this biomaterial is capable of eluting active antibiotic at concentrations that reduce bacterial growth on biomaterials and tissue, which in turn may prevent biofilm formation. Blends of chitosan and mannitol may be useful in prevention and treatment of osteomyelitis and implant-associated infections. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text