Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq
| Autor: | Zhenhua Guo, Shuhua Zhang, Chuanxue Liu, Ma Wendong, Guojun Pan, Lijun Cai, Zhiqiang Chen, Ruiying Wang, Lanming Zhang, Guan Shiwu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
chilling tolerance Candidate gene Oryza sativa RNA-Seq Biology Quantitative trait locus 01 natural sciences Transcriptome 03 medical and health sciences parasitic diseases lcsh:Science Gene 030304 developmental biology BSA-Seq Molecular breeding Genetics 0303 health sciences Multidisciplinary fungi Bulked segregant analysis candidate gene food and beverages Genetics and Genomics lcsh:Q Research Article 010606 plant biology & botany |
| Zdroj: | Royal Society Open Science Royal Society Open Science, Vol 7, Iss 11 (2020) |
| ISSN: | 2054-5703 |
| DOI: | 10.1098/rsos.201081 |
| Popis: | Rice is sensitive to low temperatures, specifically at the booting stage. Chilling tolerance of rice is a quantitative trait loci that is governed by multiple genes, and thus, its precise identification through the conventional methods is an arduous task. In this study, we investigated the candidate genes related to chilling tolerance at the booting stage of rice. The F2 population was derived from Longjing25 (chilling-tolerant) and Longjing11 (chilling-sensitive) cross. Two bulked segregant analysis pools were constructed. A 0.82 Mb region containing 98 annotated genes on chromosomes 6 and 9 was recognized as the candidate region associated with chilling tolerance of rice at the booting stage. Transcriptomic analysis of Longjing25 and Longjing11 revealed 50 differentially expressed genes (DEGs) on the candidate intervals. KEGG pathway enrichment analysis of DEGs was performed. Nine pathways were found to be enriched, which contained 10 DEGs. A total of four genes had different expression patterns or levels between Longjing25 and Longjing11. Four out of the 10 DEGs were considered as potential candidate genes for chilling tolerance. This study will assist in the cloning of the candidate genes responsible for chilling tolerance and molecular breeding of rice for the development of chilling-tolerant rice varieties. |
| Databáze: | OpenAIRE |
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