Long-term viremia and fecal shedding in pups after modified-live canine parvovirus vaccination
| Autor: | Canio Buonavoglia, Michele Losurdo, Maria Loredana Colaianni, Nicola Decaro, Stefania Rizzi, Giuseppe Crescenzo, Costantina Desario, Maria Stella Lucente, Gianpiero Ventrella, Stefano Aulicino, Alessandra Cavalli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Parvovirus Canine animal diseases viruses Viremia Antibodies Viral Vaccines Attenuated Article Parvoviridae Infections Feces Dogs medicine Animals Dog Diseases Viral shedding Seroconversion Canine parvovirus General Veterinary General Immunology and Microbiology biology Vaccination Public Health Environmental and Occupational Health Virus shedding Viral Vaccines Hemagglutination Inhibition Tests Viral Load biology.organism_classification medicine.disease Virology Titer Infectious Diseases Immunization Immunology DNA Viral Molecular Medicine Female Viral load |
| Zdroj: | Vaccine |
| ISSN: | 1873-2518 0264-410X |
| Popis: | Highlights • Dogs were administered type 2 or 2b modified-live canine parvovirus (CPV) vaccines. • Vaccine CPV-2b shedding occurred for a shorter period but with greater viral loads. • Viremia was detected for a longer period and at higher titers for CPV-2b than CPV-2. • CPV seroconvertion occurred earlier with CPV-2b than with CPV-2 vaccines. • Vaccine viruses were detected by PCR but not by antigen testing or hemagglutination. Canine parvovirus (CPV) modified live virus vaccines are able to infect vaccinated dogs replicating in the bloodstream and enteric mucosa. However, the exact duration and extent of CPV vaccine-induced viremia and fecal shedding are not known. With the aim to fill this gap, 26 dogs were administered two commercial vaccines containing a CPV-2 or CPV-2b strain and monitored for 28 days after vaccination. By using real-time PCR, vaccine-induced viremia and shedding were found to be long lasting for both vaccinal strains. Vaccinal CPV-2b shedding was detected for a shorter period than CPV-2 (12 against 19 mean days) but with greater viral loads, whereas viremia occurred for a longer period (22 against 19 mean days) and with higher titers for CPV-2b. Seroconversion appeared as early as 7 and 14 days post-vaccination for CPV-2b and CPV-2 vaccines, respectively. With no vaccine there was any diagnostic interference using in-clinic or hemagglutination test, since positive results were obtained only by fecal real-time PCR testing. The present study adds new insights into the CPV vaccine persistence in the organism and possible interference with diagnostic tests. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect