Identification and Characterization of the RNA Chaperone Activity of Hepatitis Delta Antigen Peptides
| Autor: | Zhi-Shun Huang, Huey-Nan Wu |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Molecular Sequence Data
Peptide Biochemistry Virus Substrate Specificity Antigen RNA Catalytic Amino Acid Sequence Molecular Biology Peptide sequence Hepatitis delta Antigens chemistry.chemical_classification Base Sequence biology Hydrolysis Hepatitis Antigens Ribozyme RNA Cell Biology Molecular biology chemistry biology.protein Nucleic Acid Conformation Hepatitis Delta Virus Peptides Molecular Chaperones |
| Zdroj: | Journal of Biological Chemistry. 273:26455-26461 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.273.41.26455 |
| Popis: | In this study, we identified an activity of the hepatitis delta antigen that both modulates the cis-cleaving activities of hepatitis delta virus (HDV) genomic RNA fragments and facilitates the trans-cleavage reactions between hammerhead ribozymes and the cognate substrates of various lengths in vitro. Hepatitis delta antigen peptides exert their effect by accelerating the unfolding and refolding of RNA molecules and by promoting strand annealing and strand dissociation. In addition, the stimulatory effect of hepatitis delta antigen peptide on hammerhead catalysis is observed whether the peptide is removed or not by phenol/chloroform extraction prior to the initiation of trans-cleavage reaction. Therefore, hepatitis delta antigen peptide acts as an RNA chaperone. The RNA chaperone domain of hepatitis delta antigen overlaps with the coiled-coil domain that is rich in lysine residues. The RNA binding domains of hepatitis delta antigen previously identified are not required for the RNA chaperone activity identified herein. The RNA chaperone activity of hepatitis delta antigen may be important for the regulation of HDV replication in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|