RNA silencing in the life cycle of soybean: multiple restriction systems and spatiotemporal variation associated with plant architecture
| Autor: | Akira Kanazawa, Megumi Kasai, Ayumi Mori, Hiroshi Sato, Tetsuya Yamada |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transgene Green Fluorescent Proteins Biology 01 natural sciences Green fluorescent protein 03 medical and health sciences Gene Expression Regulation Plant Genetics Gene silencing Phloem transport Gene Silencing Gene fungi food and beverages RNA Embryo Plants Genetically Modified RNA silencing 030104 developmental biology RNA Plant Seeds Animal Science and Zoology Soybeans Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Transgenic Research. 26:349-362 |
| ISSN: | 1573-9368 0962-8819 |
| DOI: | 10.1007/s11248-017-0011-8 |
| Popis: | The expression of transgenes introduced into a plant genome is sometimes suppressed by RNA silencing. Although local and systemic spread of RNA silencing have been studied, little is known about the mechanisms underlying spatial and temporal variation in transgene silencing between individual plants or between plants of different generations, which occurs seemingly stochastically. Here, we analyzed the occurrence, spread, and transmission of RNA silencing of the green fluorescent protein (GFP) gene over multiple generations of the progeny of a single soybean transformant. Observation of GFP fluorescence in entire plants of the T3-T5 generations indicated that the initiation and subsequent spread of GFP silencing varied between individuals, although this GFP silencing most frequently began in the primary leaves. In addition, GFP silencing could spread into the outer layer of seed coat tissues but was hardly detectable in the embryos. These results are consistent with the notion that transgene silencing involves its reset during reproductive phase, initiation after germination, and systemic spread in each generation. GFP silencing was absent in the pulvinus, suggesting that its cortical cells inhibit cell-to-cell spread or induction of RNA silencing. The extent of GFP silencing could differ between the stem and a petiole or between petiolules, which have limited vascular bundles connecting them and thus deter long-distant movement of silencing. Taken together, these observations indicate that the initiation and/or spread of RNA silencing depend on specific features of the architecture of the plant in addition to the mechanisms that can be conserved in higher plants. |
| Databáze: | OpenAIRE |
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