Intergenerational thyroid hormone homeostasis imbalance in cerebellum of rats perinatally exposed to glyphosate-based herbicide
| Autor: | Silvia Lima Costa, Janaina Ribeiro Pereira Soares, Eduardo B B Cunha, Roberto H. Herai, Victor Diogenes Amaral da Silva, Gisele Giannocco, Renata Marino Romano, Maria Izabel Chiamolera, Luã Tainã Costa Reis, Marco Romano, Suzana Telles da Cunha Lima, Janaina Sena de Souza, Ramon dos Santos El-Bachá, Magnus R. Dias da Silva |
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| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Cerebellum Thyroid Hormones Offspring Health Toxicology and Mutagenesis Glycine Thyroid Gland Management Monitoring Policy and Law Biology Toxicology 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Endocrine system Animals Homeostasis Rats Wistar 030304 developmental biology 0303 health sciences Thyroid hormone receptor Herbicides Thyroid General Medicine Rats Endocrinology medicine.anatomical_structure Endocrine disruptor 030220 oncology & carcinogenesis Female Hormone |
| Zdroj: | Environmental toxicologyREFERENCES. 36(6) |
| ISSN: | 1522-7278 |
| Popis: | Agrochemicals became a public health concern due to increased human exposure and possible endocrine disruption effects in several organs, including the brain. Thyroid hormones controls neurodevelopment, which turn them sensitive to endocrine disruptors (EDs). In this work, we evaluated the effect of glyphosate-based herbicides (GBH) as an intergenerational endocrine disrupter on thyroid homeostasis in cerebellar cells. Female pregnant Wistar rats were exposed to Roundup Transorb® solution at 5 and 50 mg/kg/day, from gestation day 18 to post-natal day 5 (P5). Cerebellum of male offspring was used to evaluate gene expression. The mRNA levels of thyroid hormone receptors, hormonal conversion enzymes, hormone transporters, as well as, de novo epigenetic regulators were altered, with some of these genes presenting a non-monotonic dose response. Furthermore, metabolomic profile correlation with tested dose demonstrated altered metabolic profile, in agreement with cerebellar gene alterations. Moreover, cerebellar primary cultures exposed to non-toxic GBH concentration presented a decrease level in glial fibrillary acidic protein, a protein regulated by endocrine signals. In conclusion, our results indicate that animals exposed to non-toxic GBH doses during perinatal phase carry intergenerational alterations in key regulators of cellular thyroid hormone homeostasis and epigenetic controllers in adulthood, indicating the possible ED effect of GBH based on epigenetic alterations. |
| Databáze: | OpenAIRE |
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