Cross-regulation of Hox genes in the Drosophila melanogaster embryo

Autor: Thomas C. Kaufman, Stacy L. Holtzman, Amy Kalkbrenner, Barbara Hamilton, David F.B. Miller, Bryan T. Rogers
Rok vydání: 2001
Předmět:
Central Nervous System
Transcriptional Activation
Embryology
Mesoderm
Saccharomyces cerevisiae Proteins
Time Factors
animal structures
Genotype
Transcription
Genetic
Biology
Fungal Proteins
Genes
Reporter
medicine
Animals
Drosophila Proteins
Tissue Distribution
Hox gene
Crosses
Genetic
Ultrabithorax
Plant Proteins
Homeodomain Proteins
Genetics
Regulation of gene expression
Microscopy
Confocal
Decapentaplegic
Arabidopsis Proteins
Gene Expression Regulation
Developmental
Nuclear Proteins
biology.organism_classification
Immunohistochemistry
Protein Structure
Tertiary
Cell biology
DNA-Binding Proteins
Drosophila melanogaster
medicine.anatomical_structure
Mutation
embryonic structures
Insect Proteins
Ectopic expression
Homeotic gene
Protein Binding
Signal Transduction
Transcription Factors
Developmental Biology
Zdroj: Mechanisms of Development. 102:3-16
ISSN: 0925-4773
DOI: 10.1016/s0925-4773(01)00301-x
Popis: Cross-regulation of Homeotic Complex (Hox) genes by ectopic Hox proteins during the embryonic development of Drosophila melanogaster was examined using Gal4 directed transcriptional regulation. The expression patterns of the endogenous Hox genes were analyzed to identify cross-regulation while ectopic expression patterns and timing were altered using different Gal4 drivers. We provide evidence for tissue specific interactions between various Hox genes and demonstrate the induction of endodermal labial (lab) by ectopically expressed Ultrabithorax outside the visceral mesoderm (VMS). Similarly, activation and repression of Hox genes in the VMS from outside tissues seems to be mediated by decapentaplegic (dpp) gene activation. Additionally, we find that proboscipedia (pb) is activated in the epidermis by ectopically driven Sex combs reduced (Scr) and Deformed (Dfd); however, mesodermal pb expression is repressed by ectopic Scr in this tissue. Mutant analyses demonstrate that Scr and Dfd regulate pb in their normal domains of expression during embryogenesis. Ectopic Ultrabithorax and Abdominal-A repress only lab and Scr in the central nervous system (CNS) in a timing dependent manner; otherwise, overlapping expression in the CNS in tolerated. A summary of Hox gene cross-regulation by ectopically driven Hox proteins is tabulated for embryogenesis.
Databáze: OpenAIRE
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