Characterization of poly(L-glutamic acid)-grafted hyaluronan as a novel candidate medicine and biomedical device for intra-articular injection
| Autor: | Yuuta Yanagita, Nana Hiura, Kazuaki Muramatsu, Yuya Tajima, Hiroyuki Hirai, Rin Kaneko |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Materials science Biomedical Engineering Anti-Inflammatory Agents Injections Intra-Articular Biomaterials Rats Sprague-Dawley 03 medical and health sciences Hydrolysis 0302 clinical medicine Chondrocytes Hyaluronidase In vivo medicine Animals Hyaluronic Acid Cells Cultured 030203 arthritis & rheumatology Viscosity Metals and Alloys Glutamic acid Dynamic mechanical analysis Osteoarthritis Knee Grafting In vitro Elasticity 030104 developmental biology medicine.anatomical_structure Polyglutamic Acid Ceramics and Composites Biophysics Bone marrow medicine.drug Biomedical engineering |
| Zdroj: | Journal of biomedical materials research. Part A. 105(11) |
| ISSN: | 1552-4965 |
| Popis: | A novel hyaluronan (HA) derivative, poly(L-glutamic acid)-grafted hyaluronan (PGA-g-HA), was synthesized to improve the durability of conventional HA products for intra-articular injection. The purpose of this study was to investigate the characteristics of the novel HA derivative in terms of viscoelasticity, degradation behavior, non-immunogenicity, and bioactivity using preliminary in vitro and in vivo experiments. The storage modulus (G') and loss modulus (G″) of PGA-g-HA were similar to those of HA80 (approximately 8.0 × 105 Da) rather than those of original HA200 (approximately 2.0 × 106 Da). PGA-g-HA showed strong resistance against hyaluronidase hydrolysis compared to unmodified HA200. The immunogenicity resulting from grafting PGA to HA200 was not detected in bone marrow derived dendritic cells. The anti-inflammatory activity of PGA-g-HA was confirmed in IL-1β-stimulated chondrocytes. In addition, compared to unmodified HA200, the intra-articular injection of PGA-g-HA produced greater chondroprotective effects on a monoiodoacetic acid-induced model of rat knee osteoarthritis at two weeks after a single treatment. Therefore, PGA-g-HA is expected to be a promising medicine and biomedical device for intra-articular injection. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3006-3016, 2017. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text