Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway
| Autor: | Helen K. Leech, Richard W. Pickersgill, Peter Heathcote, Andy Lawrence, Evelyne Deery, Martin J. Warren, Stefanie Frank, Amanda A. Brindley |
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| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty biology Stereochemistry Biochemistry Catalysis law.invention Vitamin B 12 chemistry.chemical_compound Enzyme chemistry Biosynthesis law Committed step Molecular genetics Recombinant DNA biology.protein medicine Bioorganic chemistry Anaerobiosis Mode of action Anaerobic exercise |
| Zdroj: | Biochemical Society Transactions. 33:811-814 |
| ISSN: | 1470-8752 0300-5127 |
| DOI: | 10.1042/bst0330811 |
| Popis: | The anaerobic biosynthesis of vitamin B12 is slowly being unravelled. Recent work has shown that the first committed step along the anaerobic route involves the sirohydrochlorin (chelation of cobalt into factor II). The following enzyme in the pathway, CbiL, methylates cobalt-factor II to give cobalt-factor III. Recent progress on the molecular characterization of this enzyme has given a greater insight into its mode of action and specificity. Structural studies are being used to provide insights into how aspects of this highly complex biosynthetic pathway may have evolved. Between cobalt-factor III and cobyrinic acid, only one further intermediate has been identified. A combination of molecular genetics, recombinant DNA technology and bioorganic chemistry has led to some recent advances in assigning functions to the enzymes of the anaerobic pathway. |
| Databáze: | OpenAIRE |
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