Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells
Autor: | Matthew Trotter, Richard Apps, Victoria Male, Ashley Moffett, N. Miller, R. North, Sandra A. Founds, Andrew M. Sharkey, Lucy Gardner |
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Jazyk: | angličtina |
Rok vydání: | 2011 |
Předmět: |
First trimester
medicine.medical_treatment Microarray Collagen receptor 0302 clinical medicine EVT extravillous trophoblast Pre-Eclampsia Pregnancy Obstetrics and Gynaecology Cluster Analysis Gene Regulatory Networks reproductive and urinary physiology Cells Cultured 0303 health sciences 030219 obstetrics & reproductive medicine Decidua Obstetrics and Gynecology Cell biology ECM extracellular matrix Trophoblasts medicine.anatomical_structure Cytokine embryonic structures VT villous trophoblast Female Chorionic Villi Adult Trophoblast differentiation Biology Article 03 medical and health sciences Fetal membrane Cell Line Tumor medicine Humans Transcription factor 030304 developmental biology uNK uterine NK Uterine NK cells Microarray analysis techniques Genome Human Gene Expression Profiling Trophoblast Microarray Analysis Gene expression profiling LAIR-2 Pregnancy Trimester First Reproductive Medicine Case-Control Studies Immunology Developmental Biology |
Zdroj: | Placenta |
ISSN: | 1532-3102 0143-4004 |
Popis: | We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray. Villous trophoblast (VT) is in contact with maternal blood and mediates nutrient exchange whereas extravillous trophoblast (EVT) invades the decidua and remodels uterine arteries. Using highly purified first trimester trophoblast we identified over 3000 transcripts that are differentially expressed. Many of these transcripts represent novel functions and pathways that show co-ordinated up-regulation in VT or EVT. In addition we identify new players in established functions such as migration, immune modulation and cytokine or angiogenic factor secretion by EVT. The transition from VT to EVT is also characterised by alterations in transcription factors such as STAT4 and IRF9, which may co-ordinate these changes. Transcripts encoding several members of the immunoglobulin-superfamily, which are normally expressed on leukocytes, were highly transcribed in EVT but not expressed as protein, indicating specific control of translation in EVT. Interactions of trophoblast with decidual leukocytes are involved in regulating EVT invasion. We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction. This represents a new mechanism by which EVT can modulate leukocyte function in the decidua. Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy. |
Databáze: | OpenAIRE |
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