Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells

Autor: Matthew Trotter, Richard Apps, Victoria Male, Ashley Moffett, N. Miller, R. North, Sandra A. Founds, Andrew M. Sharkey, Lucy Gardner
Jazyk: angličtina
Rok vydání: 2011
Předmět:
First trimester
medicine.medical_treatment
Microarray
Collagen receptor
0302 clinical medicine
EVT
extravillous trophoblast
Pre-Eclampsia
Pregnancy
Obstetrics and Gynaecology
Cluster Analysis
Gene Regulatory Networks
reproductive and urinary physiology
Cells
Cultured
0303 health sciences
030219 obstetrics & reproductive medicine
Decidua
Obstetrics and Gynecology
Cell biology
ECM
extracellular matrix
Trophoblasts
medicine.anatomical_structure
Cytokine
embryonic structures
VT
villous trophoblast
Female
Chorionic Villi
Adult
Trophoblast differentiation
Biology
Article
03 medical and health sciences
Fetal membrane
Cell Line
Tumor
medicine
Humans
Transcription factor
030304 developmental biology
uNK
uterine NK
Uterine NK cells
Microarray analysis techniques
Genome
Human
Gene Expression Profiling
Trophoblast
Microarray Analysis
Gene expression profiling
LAIR-2
Pregnancy Trimester
First
Reproductive Medicine
Case-Control Studies
Immunology
Developmental Biology
Zdroj: Placenta
ISSN: 1532-3102
0143-4004
Popis: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray. Villous trophoblast (VT) is in contact with maternal blood and mediates nutrient exchange whereas extravillous trophoblast (EVT) invades the decidua and remodels uterine arteries. Using highly purified first trimester trophoblast we identified over 3000 transcripts that are differentially expressed. Many of these transcripts represent novel functions and pathways that show co-ordinated up-regulation in VT or EVT. In addition we identify new players in established functions such as migration, immune modulation and cytokine or angiogenic factor secretion by EVT. The transition from VT to EVT is also characterised by alterations in transcription factors such as STAT4 and IRF9, which may co-ordinate these changes. Transcripts encoding several members of the immunoglobulin-superfamily, which are normally expressed on leukocytes, were highly transcribed in EVT but not expressed as protein, indicating specific control of translation in EVT. Interactions of trophoblast with decidual leukocytes are involved in regulating EVT invasion. We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction. This represents a new mechanism by which EVT can modulate leukocyte function in the decidua. Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.
Databáze: OpenAIRE
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