Expression of leptin receptor mRNA (long form splice variant) in the human cerebellum
| Autor: | Christelle Graviou, Claude Huguenin, Constantin Bouras, Armand Savioz, Brigitte Greggio, Yves Charnay |
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| Rok vydání: | 1997 |
| Předmět: |
Gene isoform
Carrier Proteins/genetics medicine.medical_specialty RNA Splicing Receptors Cell Surface In situ hybridization Biology ddc:616.89 ddc:590 Cerebellum Internal medicine Gene expression medicine Animals Humans Obesity RNA Messenger Receptors Cytokine Receptor In Situ Hybridization Cerebellum/metabolism Messenger RNA Leptin receptor Oligonucleotide General Neuroscience Receptors Cytokine/genetics Alternative splicing RNA Messenger/biosynthesis Rats Cell biology Endocrinology Receptors Leptin Carrier Proteins |
| Zdroj: | Neuroreport, Vol. 8, No 14 (1997) pp. 3123-6 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/00001756-199709290-00023 |
| Popis: | Primers specific to the long isoform of leptin receptor (OB-Rb) mRNA were used in reverse transcriptase-linked polymerase chain reactions (RT-PCR) to investigate the expression of this receptor in the hypothalamus and cerebellum of human and rat. For both regions, we observed RT-PCR cDNAs as well as restriction enzyme cleavage fragments of expected sizes. Additionally, in situ hybridization of human cerebellum using two independent [35S]oligonucleotide probes complementary to the OB-Rb mRNA sequence revealed a prominent hybridization signal within the granular layer. Overall, our findings demonstrate the expression of OB-Rb mRNA in the cerebellum and suggest that in such a location, leptin receptors may mediate a function presumably not linked to body weight homeostasis. |
| Databáze: | OpenAIRE |
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