Factors of intermittent HIV-1 excretion in semen and efficiency of sperm processing in obtaining spermatozoa without HIV-1 genomes
| Autor: | Christophe Pasquier, Louis Bujan, Jacques Izopet, Laurence Righi, Laetitia Berges, Myriam Daudin, Alain Berrebi, Laurence Thauvin, Tomohiro Matsuda, Patrice Massip |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male endocrine system Time Factors Immunology HIV Infections Semen Cell Separation Virus Replication Virus Andrology Excretion chemistry.chemical_compound Risk Factors Humans Immunology and Allergy biology urogenital system virus diseases RNA Middle Aged Viral Load biology.organism_classification Spermatozoa Virology Infectious Diseases Viral replication chemistry Lentivirus HIV-1 Tissue and Organ Harvesting RNA Viral Viral load DNA |
| Zdroj: | AIDS. 18:757-766 |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/00002030-200403260-00006 |
| Popis: | Objective To study the risk factors for HIV-1 in semen according to the localization of HIV-1 in sperm cell fractions and to assess the efficiency of sperm processing in obtaining spermatozoa without HIV-1 genomes. Methods Ninety-four HIV-infected patients provided 281 paired blood and semen samples. Sperm cell separation was performed using two successive methods. HIV-1 RNA was quantified in blood and seminal plasma. HIV-1 RNA and DNA were detected in cell fractions. Results HIV-1 RNA was found in 14% of seminal plasma samples and up to 8.7% of native semen cells were positive for HIV-1 RNA and DNA. Ten seminal plasma samples had detectable RNA although blood viral load was undetectable. Antiretroviral treatment reduced the likelihood of RNA detection in seminal plasma. For semen with polynuclear cells and HIV-1 RNA in seminal plasma, the likelihood of detecting HIV-1 genomes in semen cells was increased fourfold and sixfold, respectively. In 25% of patients, HIV-1 excretion was intermittent. In the group of patients with systematic negative seminal plasma, HIV-1 genomes were detected in up to 10% of sperm cell samples. Our method of sperm processing always enabled us to obtain spermatozoa without detectable HIV-1 genomes. Conclusions Polynuclear cells in semen are a risk factor for seminal HIV-1 excretion. Blood viral load was the only predictive factor for the intermittence of HIV-1 excretion in semen over time. Sperm processing using two successive methods was effective in obtaining spermatozoa without detectable HIV-1 genomes regardless of the viral load level in native semen. |
| Databáze: | OpenAIRE |
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