Comparative study of the control of basal acid output from rodent isolated stomachs
| Autor: | N J Welsh, James W. Black, N.P. Shankley |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
medicine.medical_specialty IBMX Phosphodiesterase Inhibitors In Vitro Techniques Biology H(+)-K(+)-Exchanging ATPase Gastric Acid Mice chemistry.chemical_compound 1-Methyl-3-isobutylxanthine Internal medicine medicine Animals Rats Wistar Phosphodiesterase inhibitor Gastrin Pharmacology Bucladesine Stomach Proton Pump Inhibitors Rats Endocrinology Histamine H2 Antagonists chemistry Gastric Mucosa Gastric acid Thiocyanates Histamine Research Article Adenylyl Cyclases medicine.drug |
| Zdroj: | British Journal of Pharmacology. 109:941-945 |
| ISSN: | 0007-1188 |
| DOI: | 10.1111/j.1476-5381.1993.tb13711.x |
| Popis: | 1. Isolated, lumen-perfused, whole stomach preparations from mouse and immature rat produced a stable basal acid output which, although not blocked by histamine H2-, acetylcholine M- or CCKB/gastrin receptor antagonists, was almostly completely blocked by the H+/K(+)-ATPase inhibitor, omeprazole, and the metabolic inhibitor, sodium thiocyanate (NaSCN). 2. Fully-defined concentration-effect curves could be obtained on both assays with the phosphodiesterase inhibitor, isobutyl methylxanthine (IBMX) and with dibutyryl cyclic AMP. 3. On the rat stomach assay, histamine H2-receptor blockade had no effect on the IBMX curve. In contrast, the IBMX response in the mouse was abolished by histamine H2-receptor blockade. On both assays responses to dibutyryl cyclic AMP were resistant to H2-receptor blockade. 4. In the absence of suprathreshold endogenous histamine, it is argued that H+/K(+)-ATPase mediated basal acid secretion from the mouse stomach assay is regulated by something other than cyclic AMP. |
| Databáze: | OpenAIRE |
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