| Autor: |
Kotaro, Matoba, Manabu, Murakami, Emi, Fujita, Shigeki, Jin, Ryosuke, Ogasawara, Tomoko, Matoba, Akiko, Takeuchi, Sanae, Haga, Michitaka, Ozaki, Hideki, Hyodoh |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Legal Medicine. 57:102071 |
| ISSN: |
1344-6223 |
| Popis: |
In forensic medicine, although various alcohols have been reported as indicators of decomposition in collected blood, no studies have examined short-chain fatty acids as indicators. In this study, the blood n-butyric acid concentration was quantified, and the association between n-butyric acid and decomposition was investigated to determine whether the detection of n-butyric acid could be a new indicator of decomposition. Among the forensic autopsies performed from 2016 to 2018 in our laboratory, the cases were divided into decomposed (n = 20) and non-decomposed (n = 20) groups based on macroscopic findings. Blood samples collected at the time of autopsy were derivatized with 3-nitrophenylhydrazine hydrochloride after solid-phase extraction. The n-butyric acid concentration was measured using liquid chromatography-tandem mass spectrometry. In addition, ethanol and n-propanol were measured using a gas chromatography-flame ionization detector. There was a significant difference (p 0.01) in the concentrations of n-butyric acid between the decomposed and non-decomposed groups (0.343 ± 0.259 [0.030-0.973] and 0.003 ± 0.002 [0.001-0.007] mg/mL, respectively). In the decomposed group, n-butyric acid was detected at high concentrations, even in cases where n-propanol was low. These results suggest that n-butyric acid is more likely to be an indicator of blood decomposition than n-propanol. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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