Cellular response to pulsed low-dose rate irradiation in X-ray sensitive hamster mutant cell lines
| Autor: | C. van Bree, Nicolaas A. P. Franken, M. Z. Zdzienicka, Hans M. Rodermond, N. Castro Kreder, Jaap Haveman, R. ten Cate |
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| Přispěvatelé: | CCA -Cancer Center Amsterdam, Radiotherapy, Faculteit der Geneeskunde |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
DNA Repair
Cell Survival DNA repair DNA damage Health Toxicology and Mutagenesis DNA Single-Stranded Hamster CHO Cells Radiation Tolerance Chinese hamster Cricetulus Cricetinae Animals Radiology Nuclear Medicine and imaging Clonogenic assay DNA Single Strand Break Radiation biology Cell Cycle Dose-Response Relationship Radiation DNA biology.organism_classification Molecular biology Non-homologous end joining Mutation Homologous recombination DNA Damage |
| Zdroj: | Journal of radiation research, 45(3), 385-391. Japan Radiation Research Society Journal of Radiation Research, 45(3), 385-391. Japan Radiation Research Society |
| ISSN: | 0449-3060 |
| Popis: | The role of DNA repair mechanisms in the cellular response to low dose rate (LDR) irradiation was studied with the aim to gain insight in the process of sublethal damage (SLD) repair. Chinese hamster cell lines mutated in either DNA single strand break (ssb) repair or DNA double strand break (dsb) repair by non homologous end joining (NHEJ) and homologous recombination (HR), or showing an AT-like phenotype, were irradiated in plateau-phase either at high dose rate (HDR, 3.3 Gy/min) or at pulsed low dose rate (p-LDR, average 1 Gy/h). Cell survival after irradiation was assessed using the clonogenic assay. A change in sensitivity when the dose rate was decreased was observed for all parental cell lines and the DNA ssb repair mutant. No difference in cell survival after p-LDR versus. HDR irradiation was observed for the two NHEJ mutants, the AT-like mutant and the HR mutant. Based on these results we conclude that single strand break repair does not play a role in the dose rate effect. The AT like protein, functional NHEJ and XRCC3 are required for the dose rate effect. |
| Databáze: | OpenAIRE |
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