Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production
| Autor: | Adrian Nikolas Mihut, Yixiao Wu, Yvonne Genzel, Wen-Song Tan, Udo Reichl, Xuping Liu, Johannes Fritsch, Thomas Bissinger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Virus Cultivation
viruses Cell Biology medicine.disease_cause Virus Replication Virus Cell Line Madin Darby Canine Kidney Cells Bioreactors Dogs Orthomyxoviridae Infections Influenza A virus medicine Doubling time Animals General Veterinary General Immunology and Microbiology Viral Vaccine Public Health Environmental and Occupational Health Embryonated Virion Virology Titer Infectious Diseases medicine.anatomical_structure Cell culture Batch Cell Culture Techniques Influenza Vaccines Molecular Medicine |
| Zdroj: | Vaccine |
| Popis: | Control and prevention of rapid influenza spread among humans depend on the availability of efficient and safe seasonal and pandemic vaccines, made primarily from inactivated influenza virus particles. Current influenza virus production processes rely heavily on embryonated chicken eggs or on cell culture as substrate for virus propagation. Today’s efforts towards process intensification in animal cell culture could innovate viral vaccine manufacturing using high-yield suspension cells in high cell density perfusion processes. In this work, we present a MDCK cell line adapted to grow as single cell suspension with a doubling time of less than 20 h, achieving cell concentrations over 1 × 107 cells/mL in batch mode. Influenza A virus titer obtained in batch infections were 3.6 log10(HAU/100 µL) for total- and 109 virions/mL for infectious virus particles (TCID50), respectively. In semi-perfusion mode concentrations up to 6 × 107 cells/mL, accumulated virus titer of 4.5 log10(HAU/100 µL) and infectious titer of almost 1010 virions/mL (TCID50) were possible. This exceeds results reported previously for cell culture-based influenza virus propagation by far and suggests perfusion cultures as the preferred method in viral vaccine manufacturing. |
| Databáze: | OpenAIRE |
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