High precision, localized proton gradients and fluxes generated by a microelectrode device induce differential growth behaviors of pollen tubes
| Autor: | Marianne Aellen, Ueli Grossniklaus, Bradley J. Nelson, Hannes Vogler, Naveen Shamsudhin, Jan T. Burri, Nino F. Läubli, Chengzhi Hu, Salvador Pané, Bumjin Jang |
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| Přispěvatelé: | University of Zurich, Hu, Chengzhi |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine 1303 Biochemistry Biomedical Engineering 2204 Biomedical Engineering 1600 General Chemistry Bioengineering Pollen Tube 580 Plants (Botany) 01 natural sciences Biochemistry Tissue Culture Techniques Double fertilization 03 medical and health sciences Bursting 10126 Department of Plant and Microbial Biology Lab-On-A-Chip Devices Extracellular Pollen tube tip 10211 Zurich-Basel Plant Science Center Electrochemical gradient 1502 Bioengineering Chemistry food and beverages Equipment Design General Chemistry Hydrogen-Ion Concentration Plant cell Microelectrode 030104 developmental biology Biophysics Pollen tube Lilium Protons Microelectrodes 010606 plant biology & botany Biomedical engineering |
| Zdroj: | Lab on a Chip |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c6lc01307d |
| Popis: | Pollen tubes are tip-growing plant cells that deliver the sperm cells to the ovules for double fertilization of the egg cell and the endosperm. Various directional cues can trigger the reorientation of pollen tube growth direction on their passage through the female tissues. Among the external stimuli, protons serve an important, regulatory role in the control of pollen tube growth. The generation of local guidance cues has been challenging when investigating the mechanisms of perception and processing of such directional triggers in pollen tubes. Here, we developed and characterized a microelectrode device to generate a local proton gradient and proton flux through water electrolysis. We confirmed that the cytoplasmic pH of pollen tubes varied with environmental pH change. Depending on the position of the pollen tube tip relative to the proton gradient, we observed alterations in the growth behavior, such as bursting at the tip, change in growth direction, or complete growth arrest. Bursting and growth arrest support the hypothesis that changes in the extracellular H+ concentration may interfere with cell wall integrity and actin polymerization at the growing tip. A change in growth direction for some pollen tubes implies that they can perceive the local proton gradient and respond to it. We also showed that the growth rate is directly correlated with the extracellular pH in the tip region. Our microelectrode approach provides a simple method to generate protons and investigate their effect on plant cell growth. |
| Databáze: | OpenAIRE |
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