The WASP Homologue Las17 Activates the Novel Actin-regulatory Activity of Ysc84 to Promote Endocytosis in Yeast
| Autor: | Kathryn R. Ayscough, Ellen G. Allwood, Fiona C. Gardiner, Alastair S. Robertson, Adam P. Smith, Rosaria Costa, Steve J. Winder |
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| Rok vydání: | 2009 |
| Předmět: |
Saccharomyces cerevisiae Proteins
biology Microfilament Proteins Endocytic cycle Wiskott–Aldrich syndrome protein Arp2/3 complex Actin remodeling Articles Saccharomyces cerevisiae macromolecular substances Cell Biology Microfilament Actins Endocytosis Cell biology Microscopy Electron Gene Expression Regulation Fungal biology.protein Actin-binding protein MDia1 Molecular Biology Gene Deletion Wiskott-Aldrich Syndrome Protein Actin Protein Binding |
| Zdroj: | Molecular Biology of the Cell. 20:1618-1628 |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e08-09-0982 |
| Popis: | Actin plays an essential role in many eukaryotic cellular processes, including motility, generation of polarity, and membrane trafficking. Actin function in these roles is regulated by association with proteins that affect its polymerization state, dynamics, and organization. Numerous proteins have been shown to localize with cortical patches of yeast actin during endocytosis, but the role of many of these proteins remains poorly understood. Here, we reveal that the yeast protein Ysc84 represents a new class of actin-binding proteins, conserved from yeast to humans. It contains a novel N-terminal actin-binding domain termed Ysc84 actin binding (YAB), which can bind and bundle actin filaments. Intriguingly, full-length Ysc84 alone does not bind to actin, but binding can be activated by a specific motif within the polyproline region of the yeast WASP homologue Las17. We also identify a new monomeric actin-binding site on Las17. Together, the polyproline region of Las17 and Ysc84 can promote actin polymerization. Using live cell imaging, kinetics of assembly and disassembly of proteins at the endocytic site were analyzed and reveal that loss of Ysc84 and its homologue Lsb3 decrease inward movement of vesicles consistent with a role in actin polymerization during endocytosis. |
| Databáze: | OpenAIRE |
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