The early response of Atlantic salmon (Salmo salar) macrophages exposed in vitro to Aeromonas salmonicida cultured in broth and in fish
| Autor: | Krista Melville, Robert C. Richards, K. Vanya Ewart, Susan E. Douglas, Jeffrey W. Gallant, Jason Williams |
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| Rok vydání: | 2007 |
| Předmět: |
Innate immune system
biology animal diseases Gene Expression Profiling Macrophages Immunology Salmo salar Virulence Aeromonas salmonicida biology.organism_classification Tryptic soy broth Microbiology chemistry.chemical_compound chemistry Gene Expression Regulation Phagocytosis In vivo Animals Salmo Pathogen Bacteria Developmental Biology Oligonucleotide Array Sequence Analysis |
| Zdroj: | Developmental and comparative immunology. 32(4) |
| ISSN: | 0145-305X |
| Popis: | Aeromonas salmonicida is a fish pathogen that causes furunculosis. Virulent strains of this bacterium are able to infect salmonid macrophages and survive within them, although mechanisms favouring intracellular survival are not completely understood. It is known that A. salmonicida cultured in vivo in the peritoneal cavity of the host undergoes changes in gene expression and surface architecture compared with cultures grown in vitro in broth. Therefore, in this study, the early macrophage responses to A. salmonicida grown in vivo and in vitro were compared. Macrophage-enriched cell preparations from head kidney of Atlantic salmon (Salmo salar) were infected in vitro in 96-well microtitre dishes and changes in gene expression during the infection process were monitored using a custom Atlantic salmon cDNA microarray. A. salmonicida cultures grown in tryptic soy broth and in peritoneal implants were used to infect the macrophages. The macrophages were harvested at 0.5, 1.0 and 2.0h after addition of the bacteria to the medium. Significant changes in gene expression were evident by microarray analysis at 2.0h post-infection in macrophages infected with broth-grown and implant-grown bacteria; however, qPCR analysis revealed earlier up-regulation of JunB and TNF-alpha in macrophages exposed to the implant-grown bacteria. Up-regulation of those genes and others is consistent with the effects of extracellular products of aeromonad bacteria on macrophages and also suggests initiation of the innate immune response. |
| Databáze: | OpenAIRE |
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