Involvement of the vascular wall in regenerative processes after CA1 ischemic neuronal death
| Autor: | Joaquín Del Río, Diana Frechilla, Pablo Salazar-Colocho |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pathology medicine.medical_specialty Ischemia Nerve Tissue Proteins Biology Hippocampus Brain ischemia chemistry.chemical_compound Developmental Neuroscience von Willebrand Factor medicine Animals Vimentin Antigens Vascular tissue Cell Proliferation Neurons Microscopy Confocal Cell Death Dentate gyrus Neurogenesis Cerebral Arteries medicine.disease Neural stem cell Vascular endothelial growth factor Vascular endothelial growth factor B Adult Stem Cells Disease Models Animal Bromodeoxyuridine nervous system chemistry Ischemic Attack Transient Quinolines Fibroblast Growth Factor 2 Gerbillinae Neuroscience Developmental Biology |
| Zdroj: | International Journal of Developmental Neuroscience. 26:541-550 |
| ISSN: | 1873-474X 0736-5748 |
| Popis: | The involvement of vascular wall in response to neuronal death was challenged here using a transient forebrain ischemia model in gerbil, which causes CA1 neuronal death and trigger neurogenesis in hippocampus. We found an important vascular reaction in CA1 5 days after ischemia evaluated by Von Willebrand factor and Vimentin immunoreactivity, as well as increased expression of angiogenic and neurogenic regulators: Vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2). Analysing the morphology and cell phenotype by confocal microscopy, we confirmed the colocalization of the neurogenic markers (bromodeoxyuridine-neuronal nuclei-TOPRO-3) in newborn cells associated to vascular walls in CA1 and dentate gyrus of hippocampus 32 days after ischemia. The results indicate that vascular tissues may participate in neurogenesis after brain ischemia, reinforce the notion that blood vessels represent a source of neuronal progenitor cells in damaged brain areas and suggest that molecular and cellular manipulation of the vascular wall may expand the possibilities of novel regenerative therapies. |
| Databáze: | OpenAIRE |
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