Factors Affecting Peptide Interactions with Surface-Bound Microgels
| Autor: | Lina Nyström, Martin Malmsten, Randi Nordström, Jane Bramhill, Mark W. Rutland, Brian R. Saunders, Rubén Álvarez-Asencio |
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| Rok vydání: | 2016 |
| Předmět: |
Polymers and Plastics
Surface Properties Acrylic Resins Bioengineering Peptide binding Peptide Microgel Surface-bound Peptide Drug delivery Confocal microscopy AFM 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials Pharmaceutical Sciences Polymer chemistry Materials Chemistry Polylysine chemistry.chemical_classification Drug Carriers Chemistry Osmolar Concentration Cationic polymerization Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology Electrostatics Farmaceutiska vetenskaper 0104 chemical sciences Molecular Weight Covalent bond Ionic strength Drug delivery Biophysics 0210 nano-technology Drug carrier Gels Protein Binding |
| Zdroj: | Repositorio Institucional del Instituto Madrileño de Estudios Avanzados en Nanociencia instname Nystrom, L, Nordstrom, R, Bramhill, J, Saunders, B R, Alvarez-Ascencio, R, Rutland, M W & Malmsten, M 2016, ' Factors Affecting Peptide Interactions with Surface-Bound Microgels ', Biomacromolecules, pp. 669-678 . https://doi.org/10.1021/acs.biomac.5b01616 |
| ISSN: | 1526-4602 |
| DOI: | 10.1021/acs.biomac.5b01616 |
| Popis: | Effects of electrostatics and peptide size on peptide interactions with surface-bound microgels were investigated with ellipsometry, confocal microscopy, and atomic force microscopy (AFM). Results show that binding of cationic poly-L-lysine (pLys) to anionic, covalently immobilized, poly(ethyl acrylate-co-methacrylic acid) microgels increased with increasing peptide net charge and microgel charge density. Furthermore, peptide release was facilitated by decreasing either microgel or peptide charge density. Analogously, increasing ionic strength facilitated peptide release for short peptides. As a result of peptide binding, the surface-bound microgels displayed pronounced deswelling and increased mechanical rigidity, the latter quantified by quantitative nanomechanical mapping. While short pLys was found to penetrate the entire microgel network and to result in almost complete charge neutralization, larger peptides were partially excluded from the microgel network, forming an outer peptide layer on the microgels. As a result of this difference, microgel flattening was more influenced by the lower Mw peptide than the higher. Peptide-induced deswelling was found to be lower for higher Mw pLys, the latter effect not observed for the corresponding microgels in the dispersed state. While the effects of electrostatics on peptide loading and release were similar to those observed for dispersed microgels, there were thus considerable effects of the underlying surface on peptide-induced microgel deswelling, which need to be considered in the design of surface-bound microgels as carriers of peptide loads, for example, in drug delivery or in functionalized biomaterials. |
| Databáze: | OpenAIRE |
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