Noradrenergic activity in rat brain during rapid eye movement sleep deprivation and rebound sleep
| Autor: | J. E. Levine, Tarja Porkka-Heiskanen, J. H. Urban, Tomi Taira, Fred W. Turek, Sabrina E. Smith, Dag Stenberg |
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| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Tyrosine 3-Monooxygenase Physiology Rapid eye movement sleep Hypothalamus Sleep REM Biology Hippocampus Gene Expression Regulation Enzymologic 03 medical and health sciences Norepinephrine 0302 clinical medicine Physiology (medical) Internal medicine medicine Animals RNA Messenger Rats Wistar Wakefulness In Situ Hybridization 030304 developmental biology Cerebral Cortex 0303 health sciences Tyrosine hydroxylase Locus Ceruleus Brain Sleep in non-human animals Frontal Lobe Rats Sleep deprivation Endocrinology Organ Specificity Catecholamine Locus coeruleus Sleep Deprivation Locus Coeruleus medicine.symptom Sleep 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | The American journal of physiology. 268(6 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | Noradrenergic locus ceruleus neurons are most active during waking and least active during rapid eye movement (REM) sleep. We expected REM sleep deprivation (REMSD) to increase norepinephrine utilization and activate the tyrosine hydroxylase (TH) gene critical for norepinephrine production. Male Wistar rats were deprived of REM sleep with the platform method. Rats were decapitated after 8, 24, or 72 h on small (REMSD) or large (control) platforms or after 8 or 24 h of rebound sleep after 72 h of the platform treatment. During the first 24 h, norepinephrine concentration, measured by high-performance liquid chromatography/electrochemical detection, was lower in the neocortex, hippocampus, and posterior hypothalamus in REMSD rats than in large-platform controls. After 72 h of REMSD, TH mRNA, measured by in situ hybridization, was increased in the locus ceruleus and norepinephrine concentrations were increased. Polygraphy showed that small-platform treatment caused effective and selective REMSD. Serum corticosterone measurement by radioimmunoassay indicated that the differences found in norepinephrine and TH mRNA were not due to differences in stress between the treatments. The novel finding of sleep deprivation-specific increase in TH gene expression indicates an important mechanism of adjusting to sleep deprivation. |
| Databáze: | OpenAIRE |
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