Active Lysogeny in Listeria Monocytogenes Is a Bacteria-Phage Adaptive Response in the Mammalian Environment
| Autor: | Jessica Mioduser, Gil Azulay, Nadejda Sigal, Ilya Borovok, Anna Pasechnek, Lev Rabinovich, Anat A. Herskovits, Tal Argov, Olga Stadnyuk |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
listeria Prophages mammalian infection medicine.disease_cause General Biochemistry Genetics and Molecular Biology Article Microbiology phage excision 03 medical and health sciences Mice 0302 clinical medicine Listeria monocytogenes Bacterial Proteins Transcription (biology) bacterial pathogen Lysogenic cycle Gene expression medicine phage Animals Bacteriophages Listeriosis lcsh:QH301-705.5 Gene Lysogeny Prophage phage transcriptional response biology active lysogeny biology.organism_classification Mice Inbred C57BL 030104 developmental biology lcsh:Biology (General) Lytic cycle Listeria phage adaptaion Female Virus Activation 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Cell Reports Cell Reports, Vol 32, Iss 4, Pp 107956-(2020) |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2020.107956 |
| Popis: | Summary Some Listeria monocytogenes (Lm) strains harbor a prophage within the comK gene, which renders it inactive. During Lm infection of macrophage cells, the prophage turns into a molecular switch, promoting comK gene expression and therefore Lm intracellular growth. During this process, the prophage does not produce infective phages or cause bacterial lysis, suggesting it has acquired an adaptive behavior suited to the pathogenic lifestyle of its host. In this study, we demonstrate that this non-classical phage behavior, named active lysogeny, relies on a transcriptional response that is specific to the intracellular niche. While the prophage undergoes lytic induction, the process is arrested midway, preventing the transcription of the late genes. Further, we demonstrate key phage factors, such as LlgA transcription regulator and a DNA replicase, that support the phage adaptive behavior. This study provides molecular insights into the adaptation of phages to their pathogenic hosts, uncovering unusual cooperative interactions. Graphical Abstract Highlights • L. monocytogenes strain 10403S harbors a prophage in its comK gene • During infection of macrophage cells, the prophage lytic pathway is induced • The phage lytic response is arrested, preventing the expression of the late genes • LlgA, the late lytic gene activator is specifically inhibited at 37°C Pasechnek at al. describe a phage transcriptional response that supports the pathogenic lifestyle of its host. |
| Databáze: | OpenAIRE |
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