Differential day-night expression of tight junction components in murine retinal pigment epithelium
| Autor: | Henk Stunnenberg, Anneke I. den Hollander, Claudia Carmone, Guoqiang Yi, Peter M.T. Deen, Dorothee Günzel, Rita Rosenthal, Elja Louer |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Period (gene) Phagocytosis Retinal Pigment Epithelium Biology Sensory disorders Donders Center for Medical Neuroscience [Radboudumc 12] Mice 03 medical and health sciences Cellular and Molecular Neuroscience All institutes and research themes of the Radboud University Medical Center 0302 clinical medicine medicine Animals Circadian rhythm KEGG Molecular Biology Tight Junction Proteins Retinal pigment epithelium Tight junction RNA Retinal Photoreceptor Cell Outer Segment Actin cytoskeleton eye diseases Sensory Systems Circadian Rhythm Cell biology Mice Inbred C57BL Ophthalmology 030104 developmental biology medicine.anatomical_structure Models Animal 030221 ophthalmology & optometry sense organs |
| Zdroj: | Experimental Eye Research, 193 |
| ISSN: | 0014-4835 |
| Popis: | Strong communication and interaction between the retinal pigment epithelium (RPE) and the photoreceptor (PR) cells is essential for vision. RPE cells are essential for supporting and maintaining PR cells by transporting nutrients, waste products and ions, and phagocytosing photoreceptor outer segments (POS). POS phagocytosis follows a circadian pattern, taking place in the morning in human, mice and other organisms. However, it remains unknown whether other RPE processes follow a daily rhythm. To study the daily rhythm of RPE cells, we isolated murine RPE cells at six different time points during a 24 h period, after which RNA was isolated and sequenced. Murine RPE flatmounts were isolated at four different time points to study daily rhythm in protein abundance and localisation. EnrichR pathway analysis resulted in 13 significantly-enriched KEGG pathways (p 0.01) of which seven showed a large number of overlapping genes. Several genes were involved in intracellular trafficking, possibly playing a role in nutrient transport, POS phagocytosis or membrane protein trafficking, with different expression patterns during the day-night cycle. Other genes were involved in actin cytoskeleton building, remodelling and crosslinking and showed a high expression in the morning, suggesting actin cytoskeleton remodelling at this time point. Finally, tight junction proteins Cldn2 and Cldn4 showed a difference in RNA and protein expression and tight junction localisation over time. Our study suggests that several important processes in the RPE follow a day-night rhythm, including intracellular trafficking, and processes involving the actin cytoskeleton and tight junctions. The differential protein localisation of Cldn2 in the RPE during the day-night cycle suggest that Cldn2 may facilitate paracellular water and sodium transport during the day. |
| Databáze: | OpenAIRE |
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