Persistent measles virus infection of mouse neural cells lacking known human entry receptors
| Autor: | J. A. P. Earle, Hani'ah Abdullah, T. A. Gardiner, Frédéric Tangy, Sara Louise Cosby |
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| Přispěvatelé: | Centre for Infection and Immunity, Queen's University [Belfast] (QUB), Centre for Vision and Vascular Science, Génomique Virale et Vaccination, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2009 |
| Předmět: |
MESH: Neurons
Fluorescent Antibody Technique Apoptosis MESH: Subacute Sclerosing Panencephalitis Virus Replication Mice 0302 clinical medicine Morbillivirus MESH: Reverse Transcriptase Polymerase Chain Reaction MESH: Animals Mononegavirales MESH: Fluorescent Antibody Technique Antigens Viral Cells Cultured Neurons 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction Brain MESH: Oligodendroglia Immunohistochemistry 3. Good health Oligodendroglia [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Neurology MESH: RNA Viral MESH: Measles RNA Viral Receptors Virus MESH: Antigens Viral MESH: Cells Cultured Histology Paramyxoviridae Virus Pathology and Forensic Medicine Measles virus MESH: Brain 03 medical and health sciences Virus antigen Antigen MESH: Mice Inbred C57BL Physiology (medical) Animals Humans MESH: Mice 030304 developmental biology MESH: Humans MESH: Apoptosis MESH: Virus Replication MESH: Immunohistochemistry biology.organism_classification MESH: Receptors Virus Virology Mice Inbred C57BL Disease Models Animal Viral replication Neurology (clinical) Subacute Sclerosing Panencephalitis MESH: Disease Models Animal MESH: Measles virus 030217 neurology & neurosurgery Measles |
| Zdroj: | Neuropathology and Applied Neurobiology Neuropathology and Applied Neurobiology, Wiley, 2009, 35 (5), pp.473-86. ⟨10.1111/j.1365-2990.2009.01023.x⟩ Neuropathology and Applied Neurobiology, 2009, 35 (5), pp.473-86. ⟨10.1111/j.1365-2990.2009.01023.x⟩ |
| ISSN: | 1365-2990 0305-1846 |
| Popis: | International audience; AIMS: Infection of the mouse central nervous system with wild type (WT) and vaccine strains of measles virus (MV) results in lack of clinical signs and limited antigen detection. It is considered that cell entry receptors for these viruses are not present on murine neural cells and infection is restricted at cell entry. METHODS: To examine this hypothesis, virus antigen and caspase 3 expression (for apoptosis) was compared in primary mixed, neural cell cultures infected in vitro or prepared from mice infected intracerebrally with WT, vaccine or rodent neuroadapted viruses. Viral RNA levels were examined in mouse brain by nested and real-time reverse transcriptase polymerase chain reaction. RESULTS: WT and vaccine strains were demonstrated for the first time to infect murine oligodendrocytes in addition to neurones despite a lack of the known MV cell receptors. Unexpectedly, the percentage of cells positive for viral antigen was higher for WT MV than neuroadapted virus in both in vitro and ex vivo cultures. In the latter the percentage of positive cells increased with time after mouse infection. Viral RNA (total and mRNA) was detected in brain for up to 20 days, while cultures were negative for caspase 3 in WT and vaccine virus infections. CONCLUSIONS: WT and vaccine MV strains can use an endogenous cell entry receptor(s) or alternative virus uptake mechanism in murine neural cells. However, viral replication occurs at a low level and is associated with limited apoptosis. WT MV mouse infection may provide a model for the initial stages of persistent MV human central nervous system infections. |
| Databáze: | OpenAIRE |
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