The lipid membrane of HIV-1 stabilizes the viral envelope glycoproteins and modulates their sensitivity to antibody neutralization
| Autor: | Patrick M. Schlievert, Jacklyn Johnson, Jon C. D. Houtman, Michael S. Zhang, Hillel Haim, Manuel G Flores, Hamid Salimi, Yunxia O'Malley |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
viruses HIV Infections HIV Antibodies Gp41 Microbiology Biochemistry Virus 03 medical and health sciences Membrane Microdomains Viral envelope Viral entry Humans Lipid bilayer Molecular Biology Lipid raft chemistry.chemical_classification 030102 biochemistry & molecular biology Protein Stability env Gene Products Human Immunodeficiency Virus virus diseases Cell Biology Virus Internalization Viral membrane Antibodies Neutralizing Cholesterol HEK293 Cells 030104 developmental biology chemistry HIV-1 Biophysics lipids (amino acids peptides and proteins) Glycoprotein |
| Zdroj: | J Biol Chem |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.ra119.009481 |
| Popis: | The envelope glycoproteins (Envs) of HIV-1 are embedded in the cholesterol-rich lipid membrane of the virus. Chemical depletion of cholesterol from HIV-1 particles inactivates their infectivity. We observed that diverse HIV-1 strains exhibit a range of sensitivities to such treatment. Differences in sensitivity to cholesterol depletion could not be explained by variation in Env components known to interact with cholesterol, including the cholesterol-recognition motif and cytoplasmic tail of gp41. Using antibody-binding assays, measurements of virus infectivity, and analyses of lipid membrane order, we found that depletion of cholesterol from HIV-1 particles decreases the conformational stability of Env. It enhances exposure of partially cryptic epitopes on the trimer and increases sensitivity to structure-perturbing treatments such as antibodies and cold denaturation. Substitutions in the cholesterol-interacting motif of gp41 induced similar effects as depletion of cholesterol. Surface-acting agents, which are incorporated into the virus lipid membrane, caused similar effects as disruption of the Env-cholesterol interaction. Furthermore, substitutions in gp120 that increased structural stability of Env (i.e. induced a “closed” conformation of the trimer) increased virus resistance to cholesterol depletion and to the surface-acting agents. Collectively, these results indicate a critical contribution of the viral membrane to the stability of the Env trimer and to neutralization resistance against antibodies. Our findings suggest that the potency of poorly neutralizing antibodies, which are commonly elicited in vaccinated individuals, may be markedly enhanced by altering the lipid composition of the viral membrane. |
| Databáze: | OpenAIRE |
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