A Comparative Study of Genome-Wide Transcriptional Profiles of Primary Hepatocytes in Collagen Sandwich and Monolayer Cultures
| Autor: | Christopher D. Lasher, T. M. Murali, Yeonhee Kim, Padmavathy Rajagopalan, Logan M. Milford |
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| Rok vydání: | 2010 |
| Předmět: |
Cell Culture Techniques
Biomedical Engineering Medicine (miscellaneous) Bioengineering Biology Article Gene expression medicine Animals Gene Cells Cultured Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Regulation of gene expression Genome Tissue Scaffolds Gene Expression Profiling Metabolism Rats Amino acid Gene expression profiling medicine.anatomical_structure Gene Expression Regulation chemistry Biochemistry Rats Inbred Lew Cell culture Hepatocyte Hepatocytes Female Collagen |
| Zdroj: | Tissue Engineering Part C: Methods. 16:1449-1460 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2010.0012 |
| Popis: | Two commonly used culture systems in hepatic tissue engineering are the collagen sandwich (CS) and monolayers of cells. In this study, genome-wide gene expression profiles of primary hepatocytes were measured over an 8-day period for each cell culture system using Affymetrix GeneChips and compared via gene set enrichment analysis to elicit biologically meaningful information at the level of gene sets. Our results demonstrate that gene expression in hepatocytes in CS cultures steadily and comprehensively diverges from that in monolayer cultures. Gene sets up-regulated in CS cultures include several associated with liver metabolic and synthesis functions, such as metabolism of lipids, amino acids, carbohydrates, and alcohol, and synthesis of bile acids. Monooxygenases such as Cytochrome-P450 enzymes do not show any change between the culture systems after 1 day, but exhibit significant up-regulation in CS cultures after 3 days in comparison to hepatocyte monolayers. These data provide insights into the up- and down-regulation of several liver-critical gene sets and their subsequent effects on liver-specific functions. These results provide a baseline for further explorations into the systems biology of engineered liver mimics. |
| Databáze: | OpenAIRE |
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