BacMam technology and its application to drug discovery
| Autor: | Thomas A. Kost, Robert S. Ames, J. Patrick Condreay |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Expert Opinion on Drug Discovery. 2:1669-1681 |
| ISSN: | 1746-045X 1746-0441 |
| DOI: | 10.1517/17460441.2.12.1669 |
| Popis: | The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to be known as BacMam gene delivery. In the field of high-throughput screening the failure of many common transient gene delivery methods in reproducibility and cell survival has caused investigators to routinely apply stable cell lines in support of cell-based assays. The ease of use, versatility, safety and economics of the BacMam system makes transient gene delivery a viable option in the high-throughput screening setting and in most instances circumvents many of the limitations of stable cell lines. Although a few pharmaceutical companies have embraced the technology, its use is poised to become more widespread with increased familiarity and the emergence of enabling products based on the BacMam system. |
| Databáze: | OpenAIRE |
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