LncRNA SAMD12-AS1 Promotes the Progression of Gastric Cancer via DNMT1/p53 Axis
| Autor: | Guang-Hui Lu, Run-Dong Shao, Zi-Yuan Liu, Hui-Meng Zhao, Gang Sun, Qun Cao |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
DNA (Cytosine-5-)-Methyltransferase 1 RNA-binding protein Biology Flow cytometry 03 medical and health sciences 0302 clinical medicine Stomach Neoplasms Cell Line Tumor Gene expression medicine Humans Cell Proliferation medicine.diagnostic_test Cancer RNA General Medicine Cell cycle medicine.disease Biomarker (cell) Gene Expression Regulation Neoplastic 030104 developmental biology 030220 oncology & carcinogenesis DNMT1 Cancer research Disease Progression RNA Long Noncoding Tumor Suppressor Protein p53 |
| Zdroj: | Archives of medical research. 52(7) |
| ISSN: | 1873-5487 |
| Popis: | Background Long noncoding RNAs (lncRNAs) are essential modulators of cancers initiation and progression via regulating gene expression and biological behaviors. LncRNA SAMD12-AS1 has been validated to promote the progression of several cancers, while its role in gastric cancer (GC) remains unclear. This study aims to explore the role of LncRNA SAMD12-AS1 in GC. Methods qRT-PCR was performed to analyze the expression of lncRNA SAMD12-AS1 in GC tissues and cell lines, with Kaplan–Meier curve analyzing the correlation between LncRNA SAMD12-AS1 and prognosis. CCK-8 assay, and flow cytometry were applied to detect GC cells proliferation, cell cycle. Binding of RNA and proteins were detected via RNA binding protein immunoprecipitation (RIP) assay. Protein levels of oncogenesis-related genes were determined via western blotting. Results SAMD12-AS1 was highly up-regulated in human gastric cancer tissues and cell lines compared to their normal counterparts. High SAMD12-AS1 expression was closely related to TNM stage, and shorter survival span of patients with GC. Moreover, SAMD12-AS1 was also found to promote the oncogenic role of GC cells via inhibiting the P53 signaling pathway. Mechanistically, SAMD12-AS1 might performed its biological roles in GC via directly interacting with DNMT1 and facilitating DNMT1 repress the P53 signaling pathway. Conclusion Our study demonstrated that SAMD12-AS1 promoted GC progression via DNMT1/P53 axis, indicating SAMD12-AS1 may be envisioned as a novel biomarker of, and therapeutic target for GC. |
| Databáze: | OpenAIRE |
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