Detection of Leptospiral DNA in the Urine of Donkeys on the Caribbean Island of Saint Kitts
| Autor: | Brittney Beigel, Michel Vandenplas, Bernard Grevemeyer, Ashutosh Verma, Ellen Cho, Arve Lee Willingham |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Veterinary medicine 040301 veterinary sciences 030106 microbiology Urine Article donkey Microbiology 0403 veterinary science 03 medical and health sciences medicine TaqMan leptospirosis waterborne disease lcsh:Veterinary medicine General Veterinary biology Zoonosis 04 agricultural and veterinary sciences zoonosis medicine.disease biology.organism_classification Leptospirosis Real-time polymerase chain reaction Herd lcsh:SF600-1100 Donkey Bacteria |
| Zdroj: | Veterinary Sciences, Vol 4, Iss 1, p 2 (2017) Veterinary Sciences; Volume 4; Issue 1; Pages: 2 Veterinary Sciences |
| ISSN: | 2306-7381 |
| DOI: | 10.3390/vetsci4010002 |
| Popis: | Leptospirosis is a global zoonosis caused by pathogenic spirochetes classified within the genus Leptospira. Leptospires live in the proximal renal tubules of reservoir or chronic carrier animals, and are shed in the urine. Naïve animals acquire infection either when they come in direct contact with a reservoir or infected animals or by exposure to environmental surface water or soil that is contaminated with their urine. In this study, urine samples from a herd of donkeys on the Caribbean island of St. Kitts were screened using a TaqMan-based real-time quantitative polymerase chain reaction (qPCR) targeting a pathogen-specific leptospiral gene, lipl32. Out of 124 clinically normal donkeys, 22 (18%) tested positive for leptospiral DNA in their urine. Water samples from two water troughs used by the donkeys were also tested, but were found to be free from leptospiral contamination. Detection of leptospiral DNA in the urine of clinically healthy donkeys may point to a role that these animals play in the maintenance of the bacteria on St. Kitts. |
| Databáze: | OpenAIRE |
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