Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability
| Autor: | Ambarish Shah, Steven M. Bishop, Hasige A. Sathish, Goldberg Deborah |
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| Rok vydání: | 2011 |
| Předmět: |
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Protein Conformation Protein Stability Chemistry Chemistry Pharmaceutical Analytical chemistry Antibodies Monoclonal Pharmaceutical Science Protein aggregation Fluorescence Light scattering Fluorescence spectroscopy High-Throughput Screening Assays Protein Aggregates Colloid Spectrometry Fluorescence Differential scanning calorimetry Scattering Radiation Static light scattering Thermal stability Biological system |
| Zdroj: | Journal of Pharmaceutical Sciences. 100:1306-1315 |
| ISSN: | 0022-3549 |
| Popis: | In this work, we describe the application of two different high-throughput screening (HTS) techniques that can be used to determine protein stability during early formulation development. Differential scanning fluorescence (DSF) and differential static light scattering (DSLS) are used to determine the conformational and colloidal stability of therapeutic monoclonal antibodies (mAbs) during thermal denaturation in a high-throughput fashion. DSF utilizes SYPRO Orange, a polarity-sensitive extrinsic fluorescent probe, to monitor protein unfolding. We found that melting temperatures determined by DSF have a linear correlation with melting temperatures of the first domain unfolding determined by differential scanning calorimetry, establishing DSF as a reliable method for measuring thermal stability. The DSLS method employs static light scattering to evaluate protein stability during thermal denaturation in a 384-well format. Overall comparison between mAb aggregation under typical accelerated stress conditions (40°C) and the thermal stability obtained by DSF and DSLS is also presented. Both of these HTS methods are cost effective with high-throughput capability and can be implemented in any laboratory. Combined with other emerging HTS techniques, DSF and DSLS could be powerful tools for mAb formulation optimization. |
| Databáze: | OpenAIRE |
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