Induction of stage-specific cell cohesion in D. discoideum by a plasma-membrane-associated moiety reactive with wheat germ agglutinin
| Autor: | Charles L. Saxe, Maurice Sussman |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
biology
Molecular mass Wheat Germ Agglutinins Cell Membrane Cell Mutant Morphogenesis biology.organism_classification General Biochemistry Genetics and Molecular Biology Dictyostelium discoideum Wheat germ agglutinin Cell biology Molecular Weight medicine.anatomical_structure Membrane Biochemistry Lectins Cell Adhesion medicine Dictyostelium Polyacrylamide gel electrophoresis |
| Zdroj: | Cell. 29:755-759 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(82)90437-8 |
| Popis: | Dictyostelium discoideum strain JC-5 is a temperature-sensitive, cohesion-defective mutant. At the restrictive temperature, aggregates are formed and develop normally to a specific late stage, but then disperse to a lawn of noncohesive cells. The defect stems from the inability of the mutant at the restrictive temperature to accumulate or retain an adequate level of a newly arisen, plasma-membrane-associated cohesive moiety during the later stages of morphogenesis. We now report that the noncohesive mutant cells can be induced to cohere at a level of greater than 99% by addition of a fraction reactive with wheat germ agglutinin and isolated from the plasma membranes of late-stage, cohesive mutant or wild-type cells. Furthermore, the cohesivities of late-stage, cohesive mutant or wild-type cells can be significantly enhanced by the same preparations but early-stage cohesive cells remain unaffected. The cohesion-inducing activity can be recovered from two regions of a one-dimensional polyacrylamide gel electropherogram following elution and renaturation. The first is associated with a single silver-staining band (apparent molecular weight 95,000). It appears de novo at a postaggregative stage, and accumulates in a thermostable fashion in the wild-type and in a thermosensitive fashion in the mutants, concurrent with the loss of cohesivity. The second is associated with a complex of silver-staining bands (apparent molecular weights 40,000-50,000). The activity of the eluate reactive with wheat germ agglutinate is destroyed within 5 min at 100 degrees C. |
| Databáze: | OpenAIRE |
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