Viral Satellites Exploit Phage Proteins to Escape Degradation of the Bacterial Host Chromosome
| Autor: | Stephanie G. Hays, Kimberley D. Seed, Fatema-Tuz Johura, Munirul Alam, Amelia C. McKitterick |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
viruses
parasitism medicine.disease_cause Transduction (genetics) 0302 clinical medicine Cholera phage 2.1 Biological and endogenous factors Bacteriophages Viral Vibrio cholerae Genetics 0303 health sciences Genome biology Bacterial Chromosomes Bacterial defense helicase Lytic cycle Medical Microbiology Infection replication satellite Immunology cholera Genome Viral Bacterial genome size Microbiology Chromosomes Article Viral Proteins 03 medical and health sciences Virology medicine Humans 030304 developmental biology Synteny Circular bacterial chromosome mobile genetic element DNA Helicases Helicase Interspersed Repetitive Sequences biology.protein Parasitology Mobile genetic elements Digestive Diseases Genome Bacterial 030217 neurology & neurosurgery |
| Zdroj: | Cell Host Microbe Cell host & microbe, vol 26, iss 4 |
| ISSN: | 1931-3128 |
| DOI: | 10.1016/j.chom.2019.09.006 |
| Popis: | Phage defense systems are often found on mobile genetic elements (MGEs), where they constitutively defend against invaders or are induced to respond to new assaults. Phage satellites, one type of MGE, are induced during phage infection to promote their own transmission, reducing phage production and protecting their hosts in the process. One such satellite in Vibrio cholerae, phage-inducible chromosomal island-like element (PLE), sabotages the lytic phage ICP1, which triggers PLE excision from the bacterial chromosome, replication, and transduction to neighboring cells. Analysis of patient stool samples from different geographic regions revealed that ICP1 has evolved to possess one of two syntenic loci encoding an SF1B-type helicase, either of which PLE exploits to drive replication. Further, loss of PLE mobilization limits anti-phage activity because of phage-mediated degradation of the bacterial genome. Our work provides insight into the unique challenges facing parasites of lytic phages and underscores the adaptions of satellites to their ever-evolving target phage. |
| Databáze: | OpenAIRE |
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