Identification of genetic evidence for dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR
| Autor: | Frank T. Hufert, Manfred Weidmann, M. Vackova, P. Schmidt, Pavel Munclinger, K. Krivanec |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Orthohantavirus viruses Hantavirus Infections Molecular Sequence Data Biology Virus Rodent Diseases Mice Virology Animals Taq Polymerase Hantavirus Genetics Arvicolinae Reverse Transcriptase Polymerase Chain Reaction virus diseases Sequence Analysis DNA biology.organism_classification Reverse transcriptase Random Amplified Polymorphic DNA Technique Reverse transcription polymerase chain reaction Muridae Real-time polymerase chain reaction Apodemus Nested polymerase chain reaction |
| Zdroj: | Journal of clinical microbiology. 43(2) |
| ISSN: | 0095-1137 |
| Popis: | A survey of 158 rodents caught in the Czech Republic identified Dobrava virus sequences closely related to that of the Dobrava virus type strain in Apodemus sylvaticus and Mus musculus rodents. The identity of A. sylvaticus was unequivocally confirmed by random amplified polymorphic DNA analysis. The data seem to indicate hantavirus spillover from Apodemus flavicollis to other rodents. |
| Databáze: | OpenAIRE |
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