SDN-1/Syndecan Acts in Parallel to the Transmembrane Molecule MIG-13 to Promote Anterior Neuroblast Migration
Autor: | Lakshmi Sundararajan, Megan L Norris, Erik A. Lundquist |
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Rok vydání: | 2015 |
Předmět: |
Biology
Investigations Syndecan 1 Animals Genetically Modified 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neuroblast Cell Movement MAB-5/Hox Neuroblast migration Genetics Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Molecular Biology Genetics (clinical) 030304 developmental biology Neurons 0303 health sciences neuronal migration Cadherin Wnt signaling pathway SDN-1/syndecan Membrane Proteins Heparan sulfate Transmembrane protein Cell biology MIG-13 Biochemistry chemistry Syndecan-1 Signal transduction Carbohydrate Epimerases 030217 neurology & neurosurgery Q neuroblasts |
Zdroj: | G3: Genes|Genomes|Genetics |
ISSN: | 2160-1836 |
Popis: | The Q neuroblasts in Caenorhabditis elegans display left-right asymmetry in their migration, with QR and descendants on the right migrating anteriorly, and QL and descendants on the left migrating posteriorly. Initial QR and QL migration is controlled by the transmembrane receptors UNC-40/DCC, PTP-3/LAR, and the Fat-like cadherin CDH-4. After initial migration, QL responds to an EGL-20/Wnt signal that drives continued posterior migration by activating MAB-5/Hox activity in QL but not QR. QR expresses the transmembrane protein MIG-13, which is repressed by MAB-5 in QL and which drives anterior migration of QR descendants. A screen for new Q descendant AQR and PQR migration mutations identified mig-13 as well as hse-5, the gene encoding the glucuronyl C5-epimerase enzyme, which catalyzes epimerization of glucuronic acid to iduronic acid in the heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs). Of five C. elegans HSPGs, we found that only SDN-1/Syndecan affected Q migrations. sdn-1 mutants showed QR descendant AQR anterior migration defects, and weaker QL descendant PQR migration defects. hse-5 affected initial Q migration, whereas sdn-1 did not. sdn-1 and hse-5 acted redundantly in AQR and PQR migration, but not initial Q migration, suggesting the involvement of other HSPGs in Q migration. Cell-specific expression studies indicated that SDN-1 can act in QR to promote anterior migration. Genetic interactions between sdn-1, mig-13, and mab-5 suggest that MIG-13 and SDN-1 act in parallel to promote anterior AQR migration and that SDN-1 also controls posterior migration. Together, our results indicate previously unappreciated complexity in the role of multiple signaling pathways and inherent left-right asymmetry in the control of Q neuroblast descendant migration. |
Databáze: | OpenAIRE |
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