T2-weighted μMRI and Evoked Potential of the Visual System Measurements During the Development of Hypomyelinated Transgenic Mice
| Autor: | M. Irene Givogri, Scott E. Fraser, Anthony T. Campagnoni, Timothy D. Hiltner, Robin S. Fisher, Russell E. Jacobs, J. Michael Tyszka, Melanie Martin, Carol Readhead, Samuel D. Reyes |
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| Rok vydání: | 2006 |
| Předmět: |
Genetically modified mouse
medicine.medical_specialty Mice Transgenic Nerve Tissue Proteins Biochemistry Mice Mice Neurologic Mutants Cellular and Molecular Neuroscience Myelin Central Nervous System Diseases In vivo Internal medicine medicine Animals Longitudinal Studies Evoked potential biology Brain Myelin Basic Protein General Medicine Magnetic Resonance Imaging Myelin basic protein Endocrinology medicine.anatomical_structure biology.protein Evoked Potentials Visual T2 weighted Neuroscience Transcription Factors |
| Zdroj: | Neurochemical Research. 32:159-165 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-006-9121-z |
| Popis: | Our objective was to follow the course of a dysmyelinating disease followed by partial recovery in transgenic mice using non-invasive high-resolution (117 x 117 x 70 microm) magnetic resonance (microMRI) and evoked potential of the visual system (VEP) techniques. We used JOE (for J37 golli overexpressing) transgenic mice engineered to overexpress golli J37, a product of the Golli-mbp gene complex, specifically in oligodendrocytes. Individual JOE transgenics and their unaffected siblings were followed from 21 until 75-days-old using non-invasive in vivo VEPs and 3D T2-weighted microMRI on an 11.7 T scanner, performing what we believe is the first longitudinal study of its kind. The microMRI data indicated clear, global hypomyelination during the period of peak myelination (21-42 days), which was partially corrected at later ages (60 days) in the JOE mice compared to controls. These microMRI data correlated well with [Campagnoni AT (1995) "Molecular biology of myelination". In: Ransom B, Kettenmann H (eds) Neuroglia--a Treatise. Oxford University Press, London, pp 555-570] myelin staining, [Campagnoni AT, Macklin WB (1988) Cellular and molecular aspects of myelin protein gene-expression. Mol Neurobiol 2:41-89] a transient intention tremor during the peak period of myelination, which abated at later ages, and [Lees MB, Brostoff SW (1984) Proteins in myelin. In: Morell (ed) Myelin. Plenum Press, New York and London, pp 197-224] VEPs which all indicated a significant delay of CNS myelin development and persistent hypomyelination in JOE mice. Overall these non-invasive techniques are capable of spatially resolving the increase in myelination in the normally developing and developmentally delayed mouse brain. |
| Databáze: | OpenAIRE |
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