Encapsulation of mesenchymal stromal cells in alginate microspheres
| Autor: | Aleksandr Yu. Petrenko, Y U Petrenko, Vedeney S. Zaikov, Natalia A. Volkova, Svetlana P. Mazur, Dmitriy N. Tarusin |
|---|---|
| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Alginate microspheres Chemistry lcsh:Biotechnology MULTIPOTENT MESENCHYMAL STROMAL CELLS ALGINATE MICROSPHERES METABOLIC ACTIVITY INDUCED DIFFERENTIATION МУЛЬТИПОТЕНТНі МЕЗЕНХіМАЛЬНі СТРОМАЛЬНі КЛіТИНИ АЛЬГіНАТНі МіКРОСФЕРИ МЕТАБОЛіЧНА АКТИВНіСТЬ іНДУКОВАНЕ ДИФЕРЕНЦіЮВАННЯ МУЛЬТИПОТЕНТНЫЕ МЕЗЕНХИМАЛЬНЫЕ СТРОМАЛЬНЫЕ КЛЕТКИ АЛЬГИНАТНЫЕ МИКРОСФЕРЫ МЕТАБОЛИЧЕСКАЯ АКТИВНОСТЬ ИНДУЦИРОВАННАЯ ДИФФЕРЕНЦИРОВКА Mesenchymal stem cell multipotent mesenchymal stromal cells Multipotent Mesenchymal Stromal Cells metabolic activity Encapsulation (networking) Cell biology 03 medical and health sciences induced differentiation 030104 developmental biology 0302 clinical medicine alginate microspheres 030220 oncology & carcinogenesis lcsh:TP248.13-248.65 Metabolic activity |
| Zdroj: | Biotechnologia Acta, Vol 9, Iss 4, Pp 58-66 (2016) |
| ISSN: | 2410-776X 2410-7751 |
| Popis: | The research aim work was a comparative study of structural and functional state and capacity for differentiation of multipotent mesenchymal stromal cells, stored at various temperatures either as cell suspensions or within alginate microspheres. Storage was carried out in a culture medium based on -MEM at temperatures of 4, 22 and 37 C in sealed cryovials. After 1, 2 and 3 days the alginate microspheres were dissolved and viability (MTT test), the attachment properties and metabolic activity (AB test) were evaluated in monolayer cell culture. It has been shown that the storage of the mesenchymal stromal cells in suspension for 3 days at the indicated temperatures resulted in a decrease of the studied parameters. Mesenchymal stromal cells after storage within alginate microspheres at 22 and 37 C showed a high viability (78 and 87%, respectively), kept the attachment properties (62 and 70%), metabolic activity (79 and 75%) and ability to differentiation. The results indicate that the mesenchymal stromal cells entrapped in alginate microspheres are more resistant to storage conditions than a suspension of mesenchymal stromal cells. Entrapment in alginate microspheres is a promising technological approach for a short-term storage of mesenchymal stromal cells at positive temperatures. Key words: m ultipotent mesenchymal stromal cells, alginate microspheres, metabolic activity, induced differentiation. Multipotent mesenchymal stromal cells (MSCs) are characterized by unique properties and high therapeutic potential. For effective application of MSCs it is necessary to develop the conditions of their storage and transportation ensuring the preservation of the viability and functional activity. A common method for cell storage is cryopreservation [1, 2]. It allows the storage of biological material during unlimited period of time, however, requires the use of special equipment and cryoprotectants which are as a rule of toxic effect [3]. For a short-term storage and transportation of cells as a cheap and technically simple alternative to deep cold (-196 C) there are widely used the positive temperatures which are below of the physiological values inherent to homoiotherms. When developing the hypothermic storage conditions the major attention is concentrated to optimizing the composition of media [4, 5, 6], leaving out of the focus the spatial arrangement of cells in a liquid medium during storage. Natural sedimentation in the dispersion over time leads to a situation when individual cells occur to be in different conditions. The last circumstance can be avoided by continuously stirring the cell suspension, increasing the viscosity of the medium or formation of a specific cell environment with their entrapment in the hydrogel, for example alginate microspheres (AMSs). The advantages of cell entrapment in hydrogel AMSs as a technological method which provides an increase in cell viability during storage have been previously identified when we studied the correlation of MSCs hypothermic storage on the compositions of the used media [7]. In present paper we set out to determine if this effect occurs without using the special media for hypothermic storage. The aim of the work was a comparative study of the structural and functional condition and ability to induce differentiation of MSCs stored at positive temperatures either in the form of cell suspensions or entrapped in alginate hydrogel microspheres. |
| Databáze: | OpenAIRE |
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