Identification and characterization of lymph organ microRNAs in red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus
| Autor: | Xiu-li Shen, Xiao-Yun Leng, Xin-Cang Li, Zhi-Qiang Du, Yan-Hui Jin |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lymphoid Tissue White spot syndrome Astacoidea Aquatic Science 03 medical and health sciences White spot syndrome virus 1 microRNA Environmental Chemistry Animals Procambarus clarkii Innate immune system biology High-Throughput Nucleotide Sequencing 04 agricultural and veterinary sciences General Medicine biology.organism_classification Actin cytoskeleton Immunity Innate Cell biology MicroRNAs 030104 developmental biology Lymphatic system Immunology 040102 fisheries 0401 agriculture forestry and fisheries Vascular smooth muscle contraction |
| Zdroj: | Fishshellfish immunology. 69 |
| ISSN: | 1095-9947 |
| Popis: | MicroRNAs (miRNAs) were important post-transcriptional regulators and played vital roles in innate immunity system of invertebrates, especially in the aspect of antivirus. In this study, using high-throughput small RNAs Illumina sequencing system, differentially expressed miRNAs (DEMs) from lymph organs in red swamp crayfish, Procambarus clarkii, infected with white spot syndrome virus, were identified. As a result, 32 known miRNAs and 7 novel miRNAs were identified in crayfish lymph organ small RNAs library of NG and WG. Among them, 7 differentially expressed miRNAs (DEMs) were predicted to be involved in the lymph organ antiviral innate immunity of P. clarkii. Besides, the results showed that putative target genes of these DEMs were related with tight junction, RNA transport, regulation of actin cytoskeleton, focal adhesion, vascular smooth muscle contraction, mRNA surveillance pathway, NOD-like receptor signaling pathway, leukocyte transendothelial migration, and protein processing in endoplasmic reticulum. These results might provide the guiding theoretical foundation for future studies about crustaceans' antiviral innate immunity. |
| Databáze: | OpenAIRE |
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