Effect of a high-protein diet on the gene expression of a trypsin-sensitive, cholecystokinin-releasing peptide (monitor peptide) in the pancreas
| Autor: | Atsuko Kondo, Hidemichi Murayama, Tohru Fushiki, Etsuro Sugimoto, Satoshi Tsuzuki |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Swine Molecular Sequence Data Peptide High-protein diet Peptide hormone Biology medicine.disease_cause Biochemistry Internal medicine Casein medicine Animals Trypsin Amino Acid Sequence RNA Messenger Growth Substances Pancreas Pancreatic Secretory Trypsin Inhibitor Chromatography High Pressure Liquid Cholecystokinin chemistry.chemical_classification Enzyme Precursors Base Sequence Body Weight Rats Inbred Strains DNA Feeding Behavior Organ Size Blotting Northern Zymogen granule Diet Rats Endocrinology Gene Expression Regulation chemistry Trypsin Inhibitor Kazal Pancreatic Intercellular Signaling Peptides and Proteins Dietary Proteins Oligonucleotide Probes medicine.drug |
| Zdroj: | European Journal of Biochemistry. 199:245-252 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | The adaptation to a high protein diet of the concentration and mRNA level of a trypsin-sensitive, cholecystokinin-releasing peptide (monitor peptide), which was proposed to be the mediator of the cholecystokinin release in response to protein intake, was investigated in the rat pancreas. Adult rats were placed on one of two isocaloric diets. One group was fed a 22% casein diet (control diet) and the other a 64% casein diet (high-protein diet) for 14 days. In order to quantify the monitor peptide separately from pancreatic secretory trypsin inhibitor (PSTI-II), which is highly similar in its amino acid and mRNA nucleotide sequences to the monitor peptide but has less cholecystokinin-releasing activity, we used specific assay methods: HPLC was used for determining the monitor peptide concentration in zymogen granules and a synthetic oligonucleotide probe for determining the mRNA of the monitor peptide in the pancreas. The concentrations in the zymogen granules and the mRNA levels in the pancreas of the two peptides increased in parallel during the adaptation to the high protein diet, indicating that these two peptides were under the same control during the adaptation. The concentration and mRNA level of the monitor peptide, which were measured after 0, 3, and 14 days, increased throughout the experiment period, as did the concentration of trypsin. This suggested that the monitor peptide and trypsin may respond to similar signals during the adaptation to a high protein diet and that this apparent coordination may facilitate the adaptation of the pancreas to the diet. |
| Databáze: | OpenAIRE |
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