Low levels of alpha-synuclein in peripheral tissues are related to clinical relapse in relapsing-remitting multiple sclerosis: a pilot cross-sectional study

Autor: Manuel Mejía, Diana P. Portales-Pérez, Ildefonso Rodriguez-Leyva, Fernando Cortés-Enríquez, Miguel Ángel Macías-Islas, Erika Chi-Ahumada, María E. Jiménez-Capdeville
Rok vydání: 2019
Předmět:
Adult
Male
medicine.medical_specialty
Biopsy
animal diseases
Pilot Projects
Inflammation
Peripheral blood mononuclear cell
Stain
Gastroenterology
Statistics
Nonparametric
Flow cytometry
Young Adult
03 medical and health sciences
Multiple Sclerosis
Relapsing-Remitting
0302 clinical medicine
Antigens
CD
Internal medicine
Giant Cells
Langhans
medicine
Humans
Lectins
C-Type
heterocyclic compounds
030212 general & internal medicine
Skin
Blood Cells
medicine.diagnostic_test
business.industry
Multiple sclerosis
Middle Aged
Flow Cytometry
medicine.disease
nervous system diseases
Staining
Peripheral
Cross-Sectional Studies
Mannose-Binding Lectins
nervous system
Neurology
alpha-Synuclein
Immunohistochemistry
Female
Steroids
Neurology (clinical)
medicine.symptom
business
Cell Nucleolus
030217 neurology & neurosurgery
Follow-Up Studies
Zdroj: Journal of the Neurological Sciences. 396:87-93
ISSN: 0022-510X
DOI: 10.1016/j.jns.2018.11.003
Popis: The protein alpha-synuclein (α-Syn) has been linked to neuroinflammatory conditions. We investigated whether the presence of α-Syn in peripheral tissues is a surrogate of brain inflammatory status in a small group of relapsing-remitting multiple sclerosis (RRMS) patients in a pilot cross-sectional study. Skin biopsies and peripheral blood were sampled from 34 healthy controls and 23 MS patients for measurement of α-Syn levels. Within the RRMS group 15 patients were in remission, and 8 patients were in the relapsing phase. The protein α-Syn was evaluated by means of immunohistochemistry and flow cytometry in skin and nucleated blood cells, respectively. In the skin, α-Syn levels were lower in relapsing MS than in the other groups, both in positive area (p = .021) and staining intensity (p = .004). In blood, the percentage of α-Syn-positive lymphocytes and monocytes were not statistically different between study groups. Moreover, the use of systemic steroids did not affect α-Syn positivity in MS-relapse patients. Finally, epidermic Langerhans cells did not stain positively for α-Syn. Overall, the levels of α-Syn positivity were lower in inflammatory relapse of RRMS patients when measured in peripheral tissues. We discuss the role of α-Syn levels in inflammation according to the obtained results.
Databáze: OpenAIRE
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