Pathology and molecular epidemiology of Mycobacterium pinnipedii tuberculosis in native New Zealand marine mammals

Autor: S. Hunter, Marian Price-Carter, Pádraig J. Duignan, Desmond M. Collins, Wendi D. Roe, Anna Kokosinska, Brett D. Gartrell, Lynn Rogers, B. Lenting, Kristene Gedye, Geoffrey W. de Lisle
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Bacterial Diseases
Male
Pathology
Marine and Aquatic Sciences
Geographical locations
0403 veterinary science
Animal Cells
Medicine and Health Sciences
Sea lion
Mammals
Molecular Epidemiology
Multidisciplinary
biology
Transmission (medicine)
Eukaryota
04 agricultural and veterinary sciences
Sea Lions
Actinobacteria
Infectious Diseases
Vertebrates
Granulomas
Medicine
Tuberculosis Diagnosis and Management
Female
Cellular Types
Research Article
DNA
Bacterial
medicine.medical_specialty
Miliary tuberculosis
Tuberculosis
Formalin fixed paraffin embedded
040301 veterinary sciences
Science
Immune Cells
Immunology
Oceania
Marine Biology
Mycobacterium
Mycobacterium tuberculosis
03 medical and health sciences
Diagnostic Medicine
medicine
Animals
Marine Mammals
Mycobacterium Infections
Molecular epidemiology
Bacteria
Organisms
Biology and Life Sciences
Mycobacterium pinnipedii
Cell Biology
biology.organism_classification
medicine.disease
Tropical Diseases
Miliary Tuberculosis
030104 developmental biology
Amniotes
Earth Sciences
Cetacea
People and places
Mycobacterium Tuberculosis
New Zealand
Zdroj: PLoS ONE
PLoS ONE, Vol 14, Iss 2, p e0212363 (2019)
ISSN: 1932-6203
Popis: Mycobacterium pinnipedii causes tuberculosis in a number of pinniped species, and transmission to cattle and humans has been reported. The aims of this study were to: characterize the pathology and prevalence of tuberculosis in New Zealand marine mammals; use molecular diagnostic methods to confirm and type the causal agent; and to explore relationships between type and host characteristics. Tuberculosis was diagnosed in 30 pinnipeds and one cetacean. Most affected pinnipeds had involvement of the pulmonary system, supporting inhalation as the most common route of infection, although ingestion was a possible route in the cetacean. PCR for the RD2 gene confirmed M. pinnipedii as the causal agent in 23/31 (74%) cases (22 using DNA from cultured organisms, and one using DNA from formalin-fixed paraffin-embedded (FFPE) tissue), including the first published report in a cetacean. RD2 PCR results were compared for 22 cases where both cultured organisms and FFPE tissues were available, with successful identification of M. pinnipedii in 7/22 (31.8%). In cases with moderate to large numbers of acid-fast bacilli, RD2 PCR on FFPE tissue provided a rapid, inexpensive method for confirming M. pinnipedii infection without the need for culture. VNTR typing distinguished New Zealand M. pinnipedii isolates from M. pinnipedii isolated from Australian pinnipeds and from common types of M. bovis in New Zealand. Most (16/18) M. pinnipedii isolates from New Zealand sea lions were one of two common VNTR types whereas the cetacean isolate was a type detected previously in New Zealand cattle.
Databáze: OpenAIRE
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