A differential scanning calorimetry study of the interaction of Lasalocid antibiotic with phospholipid bilayers
| Autor: | Pierre Peretti, C Betrencourt, Cécile Grabielle-Madelmont, Michel Ollivon, A Tahir |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Lasalocid
1 2-Dipalmitoylphosphatidylcholine Calorimetry Differential Scanning Vesicle Lipid Bilayers Organic Chemistry technology industry and agriculture Ionophore Phospholipid Analytical chemistry Biological Transport Cell Biology Models Biological Biochemistry Anti-Bacterial Agents chemistry.chemical_compound Membrane Differential scanning calorimetry chemistry Dipalmitoylphosphatidylcholine Biophysics lipids (amino acids peptides and proteins) Lipid bilayer phase behavior Molecular Biology |
| Zdroj: | Chemistry and Physics of Lipids. 103:57-65 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/s0009-3084(99)00091-2 |
| Popis: | Interaction of lasalocid sodium salt (Las-Na) with dipalmitoylphosphatidylcholine (DPPC) as a membrane model was investigated by highly-sensitive differential scanning calorimetry (DSC). The insertion properties of the antibiotic were studied both in multilamellar suspensions and unilamellar vesicles, for Las-Na/DPPC molar ratios (r) ranging from 0.005 to 0.1. The effect of the antibiotic on the lipid thermotropic behavior is concentration dependent and drastically changes at a critical r of 0.04 in both model membranes. Below this ratio, Las-Na molecules interact with DPPC bilayers without disrupting the global organization of the membrane. In the multilamellar systems only the transition cooperativity is affected whereas for the mixed vesicles, a decrease in the enthalpy change suggests a different mode of insertion. Above this ratio, implantation of the antibiotic give rise to lateral phase separation in multilamellar systems. These structural modifications have repercussions on the formation of mixed LAS-Na/DPPC vesicles which seems limited to an r value of 0.04. |
| Databáze: | OpenAIRE |
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