A genetic map of tetraploid Paspalum notatum Flügge (bahiagrass) based on single-dose molecular markers

Autor: Lorena Adelina Siena, Camilo Luis Quarin, María Pía Rodríguez, Juan Pablo A. Ortiz, Juliana Stein, Silvina Claudia Pessino, Eric Javier Martínez
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Zdroj: RepHipUNR (UNR)
Universidad Nacional de Rosario
instacron:UNR
DOI: 10.1007/s11032-007-9083-0
Popis: Paspalum notatum Flugge is a warm-season forage grass with mainly diploid (2n =20) and autotetraploid (2n = 40) representa-tives.Diploid races reproduce sexually andrequire crosspollination due to a self-incom-patible mating system, while autotetraploids reproduce by aposporous apomixis. The objec-tives of this work were to develop a genetic linkage map of Paspalum notatum Flugge at the tetraploid level, identify the linkage/s group/s associated with apomixis and carry out a general characterization of its mode of inheritance. A pseudo test-cross F1 family of 113 individuals segregating for the mode of reproduction was obtained by crossinga syn-thetic completely sexual tetraploid plant (Q4188) as female parent with a naturalaposporous individual (Q4117) as pollen donor.Map construction was based on single-dose markers (SDAFs) segregating from both par-ents. Two linkage maps (female and male) were constructed. Within each map, homolo-gous groups were assembled by detectingrepulsion-phase linked SDAFs. Putative Q4188 and Q4117 homolog groups were identified by mapping shared single dose mark-ers (BSDF). The Q4188 map consisted of 263 markers distributed on 26 co-segregation groups over a total genetic distance of 1.590.6 cM, while the Q4117 map contained 216 loci dispersed on 39 co-segregation groups along 2.265.7 cM, giving an estimated genome cov-erage of 88% and 83%, respectively. Seven and 12 putative homologous chromosomes were detected within Q4188 and Q4117 maps,respectively. Afterward, ten female and male homologous chromosomes were identified by mapping BSDFs. In the Q4117 map, a single linkage group was associated with apospory. It was characterized by restriction in recombina-tion and preferential chromosome pairing. A BPSD marker mapping within this group allowed the detection of the female homolog and the putative four male groups of the set carrying apospory. Fil: Stein, Juliana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martínez, Eric Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Rodriguez Romero, María Pía. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quarin, Camilo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Databáze: OpenAIRE
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