Comparative study between the Light Cycler® and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations
| Autor: | Atia W. Sheereen, Layla A. Mammo, Jalal Saour, Tanya M. Saou’r |
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| Rok vydání: | 2006 |
| Předmět: |
Reproducibility
biology business.industry DNA Mutational Analysis Clinical Biochemistry Factor V Reproducibility of Results Light cycler General Medicine Factor ii medicine.disease Polymerase Chain Reaction Molecular biology biology.protein Factor V Leiden medicine Humans Prothrombin Restriction fragment length polymorphism Nuclear medicine business Polymorphism Restriction Fragment Length Mathematics |
| Zdroj: | Clinical Biochemistry. 39:767-769 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2006.01.019 |
| Popis: | Objectives To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice. Design and methods We compared conformity, reproducibility, speed and cost using the Light Cycler (LC®) and PCR-RFLP. Results There was 100% conformity and reproducibility. LC® was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC® testing becomes cheaper. Conclusion In our practice, LC® provides fast, reproducible and cost-effective results. |
| Databáze: | OpenAIRE |
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